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• W2040370409 endingPage "7282" @default.
• W2040370409 startingPage "7275" @default.
• W2040370409 abstract "Protein 4.1R is the prototypical member of a protein family that includes 4.1G, 4.1B, and 4.1N. 4.1R plays a crucial role in maintaining membrane mechanical integrity by binding cooperatively to spectrin and actin through its spectrin-actin-binding (SAB) domain. While the binary interaction between 4.1R and spectrin has been well characterized, the actin binding site in 4.1R remains unidentified. Moreover, little is known about the interaction of 4.1R homologues with spectrin and actin. In the present study, we showed that the 8 aa motif (LKKNFMES) within the 10 kDa spectrin-actin-binding domain of 4.1R plays a critical role in binding of 4.1R to actin. Recombinant 4.1R SAB domain peptides with mutations in this motif showed a marked decrease in their ability to form ternary complexes with spectrin and actin. Binary protein-protein interaction studies revealed that this decrease resulted from the inability of mutant SAB peptides to bind to actin filaments while affinity for spectrin was unchanged. We also documented that the 14 C-terminal residues of the 21 amino acid cassette encoded by exon 16 in conjunction with residues 27−43 encoded by exon 17 constituted a fully functional minimal spectrin-binding motif. Finally, we showed that 4.1N SAB domain was unable to form a ternary complex with spectrin and actin, while 4.1G and 4.1B SAB domains were able to form such a complex but less efficiently than 4.1R SAB. This was due to a decrease in the ability of 4.1G and 4.1B SAB domain to interact with actin but not with spectrin. These data enabled us to propose a model for the 4.1R-spectrin-actin ternary complex which may serve as a general paradigm for regulation of spectrin-based cytoskeleton interaction in various cell types." @default.
• W2040370409 created "2016-06-24" @default.
• W2040370409 creator A5034746599 @default.
• W2040370409 creator A5042844951 @default.
• W2040370409 creator A5058293120 @default.
• W2040370409 creator A5072805788 @default.
• W2040370409 date "2002-05-11" @default.
• W2040370409 modified "2023-09-23" @default.
• W2040370409 title "Functional Characterization of Spectrin-Actin-Binding Domains in 4.1 Family of Proteins" @default.
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• W2040370409 doi "https://doi.org/10.1021/bi0256330" @default.
• W2040370409 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12044158" @default.
• W2040370409 hasPublicationYear "2002" @default.
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