FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040371864> ?p ?o ?g. }

• W2040371864 endingPage "288" @default.
• W2040371864 startingPage "278" @default.
• W2040371864 abstract "Microvascular endothelial cell dysfunction plays a key role in myocardial ischemia/reperfusion (I/R) injury, wherein reactive oxygen species (ROS)-dependent signaling is intensively involved. However, the roles of the various ROS sources remain unclear. This study sought to investigate the role of NADPH oxidase 4 (Nox4) in the cardiac microvascular endothelium in response to I/R injury. Adult rat cardiac microvascular endothelial cells (CMECs) were isolated and subjected to hypoxia/reoxygenation (H/R). Our results showed that Nox4 was highly expressed in CMECs, was significantly increased at both mRNA and protein levels after H/R injury, and contributed to H/R-stimulated increase in Nox activity and ROS generation. Downregulation of Nox4 by small interfering RNA transfection did not affect cell viability or ROS production under normoxia, but exacerbated H/R injury as evidenced by increased apoptosis and inhibited cell survival, migration, and angiogenesis after H/R. Nox4 inhibition also increased prolyl hydroxylase 2 (PHD2) expression and blocked H/R-induced increases in HIF-1α and VEGF expression. Pretreatment with DMOG, a specific competitive PHD inhibitor, upregulated HIF-1α and VEGF expression and significantly reversed Nox4 knockdown-induced injury. However, Nox2 was scarcely expressed and played a minimal role in CMEC survival and angiogenesis after H/R, though a modest upregulation of Nox2 was observed. In conclusion, this study demonstrated a previously unrecognized protective role of Nox4, a ROS-generating enzyme and the major Nox isoform in CMECs, against H/R injury by inhibiting apoptosis and promoting migration and angiogenesis via a PHD2-dependent upregulation of HIF-1/VEGF proangiogenic signaling." @default.
• W2040371864 created "2016-06-24" @default.
• W2040371864 creator A5022170827 @default.
• W2040371864 creator A5041571735 @default.
• W2040371864 creator A5051382165 @default.
• W2040371864 creator A5077895438 @default.
• W2040371864 creator A5090150518 @default.
• W2040371864 date "2014-04-01" @default.
• W2040371864 modified "2023-10-14" @default.
• W2040371864 title "NADPH oxidase 4 promotes cardiac microvascular angiogenesis after hypoxia/reoxygenation in vitro" @default.
• W2040371864 cites W1965216303 @default.
• W2040371864 cites W1968996006 @default.
• W2040371864 cites W1971215115 @default.
• W2040371864 cites W1976480176 @default.
• W2040371864 cites W1984797661 @default.
• W2040371864 cites W1985569136 @default.
• W2040371864 cites W1986948325 @default.
• W2040371864 cites W1988087076 @default.
• W2040371864 cites W1998073026 @default.
• W2040371864 cites W1998919881 @default.
• W2040371864 cites W2005729255 @default.
• W2040371864 cites W2007418718 @default.
• W2040371864 cites W2009812264 @default.
• W2040371864 cites W2026587757 @default.
• W2040371864 cites W2035573195 @default.
• W2040371864 cites W2056852634 @default.
• W2040371864 cites W2057039546 @default.
• W2040371864 cites W2064549454 @default.
• W2040371864 cites W2071919212 @default.
• W2040371864 cites W2074985423 @default.
• W2040371864 cites W2075479208 @default.
• W2040371864 cites W2085128107 @default.
• W2040371864 cites W2092201740 @default.
• W2040371864 cites W2092759252 @default.
• W2040371864 cites W2102202839 @default.
• W2040371864 cites W2111609516 @default.
• W2040371864 cites W2113100031 @default.
• W2040371864 cites W2122644092 @default.
• W2040371864 cites W2124007377 @default.
• W2040371864 cites W2125435853 @default.
• W2040371864 cites W2132660227 @default.
• W2040371864 cites W2133093019 @default.
• W2040371864 cites W2139923937 @default.
• W2040371864 cites W2140442678 @default.
• W2040371864 cites W2148030708 @default.
• W2040371864 cites W2149457735 @default.
• W2040371864 cites W2150332104 @default.
• W2040371864 cites W2150459982 @default.
• W2040371864 cites W2154283765 @default.
• W2040371864 cites W2157554079 @default.
• W2040371864 cites W2163069245 @default.
• W2040371864 cites W2169236628 @default.
• W2040371864 cites W2170069804 @default.
• W2040371864 cites W2170338419 @default.
• W2040371864 cites W96597366 @default.
• W2040371864 doi "https://doi.org/10.1016/j.freeradbiomed.2014.01.027" @default.
• W2040371864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24480752" @default.
• W2040371864 hasPublicationYear "2014" @default.
• W2040371864 type Work @default.
• W2040371864 sameAs 2040371864 @default.
• W2040371864 citedByCount "39" @default.
• W2040371864 countsByYear W20403718642014 @default.
• W2040371864 countsByYear W20403718642015 @default.
• W2040371864 countsByYear W20403718642016 @default.
• W2040371864 countsByYear W20403718642017 @default.
• W2040371864 countsByYear W20403718642018 @default.
• W2040371864 countsByYear W20403718642019 @default.
• W2040371864 countsByYear W20403718642020 @default.
• W2040371864 countsByYear W20403718642022 @default.
• W2040371864 countsByYear W20403718642023 @default.
• W2040371864 crossrefType "journal-article" @default.
• W2040371864 hasAuthorship W2040371864A5022170827 @default.
• W2040371864 hasAuthorship W2040371864A5041571735 @default.
• W2040371864 hasAuthorship W2040371864A5051382165 @default.
• W2040371864 hasAuthorship W2040371864A5077895438 @default.
• W2040371864 hasAuthorship W2040371864A5090150518 @default.
• W2040371864 hasConcept C104317684 @default.
• W2040371864 hasConcept C123012128 @default.
• W2040371864 hasConcept C127561419 @default.
• W2040371864 hasConcept C173396325 @default.
• W2040371864 hasConcept C185592680 @default.
• W2040371864 hasConcept C190283241 @default.
• W2040371864 hasConcept C202751555 @default.
• W2040371864 hasConcept C22615655 @default.
• W2040371864 hasConcept C2779719074 @default.
• W2040371864 hasConcept C2779765511 @default.
• W2040371864 hasConcept C2780394083 @default.
• W2040371864 hasConcept C48349386 @default.
• W2040371864 hasConcept C502942594 @default.
• W2040371864 hasConcept C54009773 @default.
• W2040371864 hasConcept C55493867 @default.
• W2040371864 hasConcept C86803240 @default.
• W2040371864 hasConcept C95444343 @default.
• W2040371864 hasConceptScore W2040371864C104317684 @default.
• W2040371864 hasConceptScore W2040371864C123012128 @default.
• W2040371864 hasConceptScore W2040371864C127561419 @default.
• W2040371864 hasConceptScore W2040371864C173396325 @default.
• W2040371864 hasConceptScore W2040371864C185592680 @default.

• next