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• W2040372327 abstract "Abstract The 5.4 kb RNA enriched in mRNA coding for the large subunits of vitellogenin (VgL mRNA) in the gypsy moth ( Lymantria dispar ) was purified, and used to construct a cDNA library. One of the clones, pVL-80, had sequences representing an internal region of VgL mRNA. The cDNA representing the 5′-terminal region of VgL mRNA was isolated by 5′-RACE (Rapid Amplification of cDNA Ends), and found to contain sequences encoding the N-terminal amino acid sequence of Vg 190. The insert in pVL-80 was 822 bp long and had an open reading frame (ORF) extending beyond the insert in both directions. In vitro expression of the ORF produced a 32 kDa protein which reacted with Vg 190 antiserum. Southern analysis of gypsy moth genomic DNA suggested that a single copy of the VgL gene is present per genome. The pVL-80 was demonstrated to be useful as a specific and sensitive probe to investigate developmental and juvenile hormone regulation of VgL mRNA in the fat body." @default.
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• W2040372327 date "1994-09-01" @default.
• W2040372327 modified "2023-09-27" @default.
• W2040372327 title "cDNA cloning and expression of the large subunit of juvenile hormone-regulated vitellogenin from Lymantria dispar" @default.
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• W2040372327 doi "https://doi.org/10.1016/0022-1910(94)90011-6" @default.
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