Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040373824> ?p ?o ?g. }
- W2040373824 endingPage "713" @default.
- W2040373824 startingPage "679" @default.
- W2040373824 abstract "The voltage-dependent gating mechanism of KAT1 inward rectifier potassium channels was studied using single channel current recordings from Xenopus oocytes injected with KAT1 mRNA. The inward rectification properties of KAT1 result from an intrinsic gating mechanism in the KAT1 channel protein, not from pore block by an extrinsic cation species. KAT1 channels activate with hyperpolarizing potentials from −110 through −190 mV with a slow voltage-dependent time course. Transitions before first opening are voltage dependent and account for much of the voltage dependence of activation, while transitions after first opening are only slightly voltage dependent. Using burst analysis, transitions near the open state were analyzed in detail. A kinetic model with multiple closed states before first opening, a single open state, a single closed state after first opening, and a closed-state inactivation pathway accurately describes the single channel and macroscopic data. Two mutations neutralizing charged residues in the S4 region (R177Q and R176L) were introduced, and their effects on single channel gating properties were examined. Both mutations resulted in depolarizing shifts in the steady state conductance–voltage relationship, shortened first latencies to opening, decreased probability of terminating bursts, and increased burst durations. These effects on gating were well described by changes in the rate constants in the kinetic model describing KAT1 channel gating. All transitions before the open state were affected by the mutations, while the transitions after the open state were unaffected, implying that the S4 region contributes to the early steps in gating for KAT1 channels." @default.
- W2040373824 created "2016-06-24" @default.
- W2040373824 creator A5051410023 @default.
- W2040373824 creator A5062919262 @default.
- W2040373824 date "1998-12-01" @default.
- W2040373824 modified "2023-10-18" @default.
- W2040373824 title "Voltage-dependent Gating of Single Wild-Type and S4 Mutant KAT1 Inward Rectifier Potassium Channels" @default.
- W2040373824 cites W1233451781 @default.
- W2040373824 cites W1777273393 @default.
- W2040373824 cites W1947481567 @default.
- W2040373824 cites W1966667742 @default.
- W2040373824 cites W1967297467 @default.
- W2040373824 cites W1969870358 @default.
- W2040373824 cites W1970078484 @default.
- W2040373824 cites W1974997331 @default.
- W2040373824 cites W1978889370 @default.
- W2040373824 cites W1980221458 @default.
- W2040373824 cites W1985940938 @default.
- W2040373824 cites W1988727405 @default.
- W2040373824 cites W1996437700 @default.
- W2040373824 cites W1997511216 @default.
- W2040373824 cites W1998433450 @default.
- W2040373824 cites W2000054304 @default.
- W2040373824 cites W2003056297 @default.
- W2040373824 cites W2004736426 @default.
- W2040373824 cites W2005178989 @default.
- W2040373824 cites W2009518885 @default.
- W2040373824 cites W2009667219 @default.
- W2040373824 cites W2010973571 @default.
- W2040373824 cites W2012619013 @default.
- W2040373824 cites W2012787015 @default.
- W2040373824 cites W2013413397 @default.
- W2040373824 cites W2014158880 @default.
- W2040373824 cites W2014626166 @default.
- W2040373824 cites W2015426350 @default.
- W2040373824 cites W2015724584 @default.
- W2040373824 cites W2019562175 @default.
- W2040373824 cites W2021438486 @default.
- W2040373824 cites W2024003161 @default.
- W2040373824 cites W2025852280 @default.
- W2040373824 cites W2025938475 @default.
- W2040373824 cites W2031812418 @default.
- W2040373824 cites W2032539806 @default.
- W2040373824 cites W2038321772 @default.
- W2040373824 cites W2042183734 @default.
- W2040373824 cites W2043805877 @default.
- W2040373824 cites W2044097680 @default.
- W2040373824 cites W2044813037 @default.
- W2040373824 cites W2046357793 @default.
- W2040373824 cites W2053351731 @default.
- W2040373824 cites W2055252729 @default.
- W2040373824 cites W2056257770 @default.
- W2040373824 cites W2057610250 @default.
- W2040373824 cites W2058606373 @default.
- W2040373824 cites W2060984264 @default.
- W2040373824 cites W2062024310 @default.
- W2040373824 cites W2063840208 @default.
- W2040373824 cites W2067854932 @default.
- W2040373824 cites W2074705578 @default.
- W2040373824 cites W2074792101 @default.
- W2040373824 cites W2075834102 @default.
- W2040373824 cites W2080355249 @default.
- W2040373824 cites W2081784613 @default.
- W2040373824 cites W2082045257 @default.
- W2040373824 cites W2083517299 @default.
- W2040373824 cites W2083584165 @default.
- W2040373824 cites W2084386763 @default.
- W2040373824 cites W2088526093 @default.
- W2040373824 cites W2088734315 @default.
- W2040373824 cites W2089761854 @default.
- W2040373824 cites W2090091933 @default.
- W2040373824 cites W2092196529 @default.
- W2040373824 cites W2092552227 @default.
- W2040373824 cites W2093768785 @default.
- W2040373824 cites W2095952083 @default.
- W2040373824 cites W2099975374 @default.
- W2040373824 cites W2104759180 @default.
- W2040373824 cites W2105230499 @default.
- W2040373824 cites W2114674984 @default.
- W2040373824 cites W2126218195 @default.
- W2040373824 cites W2128420761 @default.
- W2040373824 cites W2128520791 @default.
- W2040373824 cites W2138270253 @default.
- W2040373824 cites W2138489954 @default.
- W2040373824 cites W2140287085 @default.
- W2040373824 cites W2143070723 @default.
- W2040373824 cites W2147323143 @default.
- W2040373824 cites W2148112832 @default.
- W2040373824 cites W2151503017 @default.
- W2040373824 cites W2156395564 @default.
- W2040373824 cites W2165830038 @default.
- W2040373824 cites W2178308981 @default.
- W2040373824 cites W2195816025 @default.
- W2040373824 doi "https://doi.org/10.1085/jgp.112.6.679" @default.
- W2040373824 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2229449" @default.
- W2040373824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9834140" @default.
- W2040373824 hasPublicationYear "1998" @default.
- W2040373824 type Work @default.