FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040384050> ?p ?o ?g. }

• W2040384050 endingPage "870" @default.
• W2040384050 startingPage "863" @default.
• W2040384050 abstract "Abstract Mutant R96H is a classic temperature‐sensitive mutant of bacteriophage T4 lysozyme. It was in fact the first variant of the protein to be characterized structurally. Subsequently, it has been studied extensively by a variety of experimental and computational techniques, but the reasons for the loss of stability of the mutant protein remain controversial. In the crystallographic refinement of the mutant structure at 1.9 Å resolution one of the bond angles at the site of substitution appeared to be distorted by about 11 ° , and it was suggested that this steric strain was one of the major factors in destabilizing the mutant. Different computationally‐derived models of the mutant structure, however, did not show such distortion. To determine the geometry at the site of mutation more reliably, we have extended the resolution of the data and refined the wildtype (WT) and mutant structures to be better than 1.1 Å resolution. The high‐resolution refinement of the structure of R96H does not support the bond angle distortion seen in the 1.9 Å structure determination. At the same time, it does confirm other manifestations of strain seen previously including an unusual rotameric state for His96 with distorted hydrogen bonding. The rotamer strain has been estimated as about 0.8 kcal/mol, which is about 25% of the overall reduction in stability of the mutant. Because of concern that contacts from a neighboring molecule in the crystal might influence the geometry at the site of mutation we also constructed and analyzed supplemental mutant structures in which this crystal contact was eliminated. High‐resolution refinement shows that the crystal contacts have essentially no effect on the conformation of Arg96 in WT or on His96 in the R96H mutant." @default.
• W2040384050 created "2016-06-24" @default.
• W2040384050 creator A5014217022 @default.
• W2040384050 creator A5053627320 @default.
• W2040384050 creator A5055253170 @default.
• W2040384050 date "2009-04-21" @default.
• W2040384050 modified "2023-10-01" @default.
• W2040384050 title "Evaluation at atomic resolution of the role of strain in destabilizing the temperature-sensitive T4 lysozyme mutant Arg 96 → His" @default.
• W2040384050 cites W1491774600 @default.
• W2040384050 cites W1710565104 @default.
• W2040384050 cites W1971251199 @default.
• W2040384050 cites W1973009732 @default.
• W2040384050 cites W1983638771 @default.
• W2040384050 cites W1993941879 @default.
• W2040384050 cites W1995017064 @default.
• W2040384050 cites W1996607909 @default.
• W2040384050 cites W1999590520 @default.
• W2040384050 cites W2001641653 @default.
• W2040384050 cites W2022277606 @default.
• W2040384050 cites W2026339550 @default.
• W2040384050 cites W2028231353 @default.
• W2040384050 cites W2035834851 @default.
• W2040384050 cites W2038840577 @default.
• W2040384050 cites W2045100030 @default.
• W2040384050 cites W2048117183 @default.
• W2040384050 cites W2053668583 @default.
• W2040384050 cites W2057984729 @default.
• W2040384050 cites W2060240148 @default.
• W2040384050 cites W2067454993 @default.
• W2040384050 cites W2076805293 @default.
• W2040384050 cites W2078933151 @default.
• W2040384050 cites W2094445728 @default.
• W2040384050 cites W2116274509 @default.
• W2040384050 cites W2116275717 @default.
• W2040384050 cites W2122109248 @default.
• W2040384050 cites W2134541062 @default.
• W2040384050 cites W2149315921 @default.
• W2040384050 cites W2167938143 @default.
• W2040384050 cites W4210675630 @default.
• W2040384050 doi "https://doi.org/10.1002/pro.93" @default.
• W2040384050 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2771290" @default.
• W2040384050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19384984" @default.
• W2040384050 hasPublicationYear "2009" @default.
• W2040384050 type Work @default.
• W2040384050 sameAs 2040384050 @default.
• W2040384050 citedByCount "12" @default.
• W2040384050 countsByYear W20403840502013 @default.
• W2040384050 countsByYear W20403840502016 @default.
• W2040384050 countsByYear W20403840502017 @default.
• W2040384050 countsByYear W20403840502019 @default.
• W2040384050 countsByYear W20403840502021 @default.
• W2040384050 countsByYear W20403840502023 @default.
• W2040384050 crossrefType "journal-article" @default.
• W2040384050 hasAuthorship W2040384050A5014217022 @default.
• W2040384050 hasAuthorship W2040384050A5053627320 @default.
• W2040384050 hasAuthorship W2040384050A5055253170 @default.
• W2040384050 hasBestOaLocation W20403840501 @default.
• W2040384050 hasConcept C104317684 @default.
• W2040384050 hasConcept C105702510 @default.
• W2040384050 hasConcept C112887158 @default.
• W2040384050 hasConcept C115624301 @default.
• W2040384050 hasConcept C12554922 @default.
• W2040384050 hasConcept C143065580 @default.
• W2040384050 hasConcept C147597530 @default.
• W2040384050 hasConcept C178790620 @default.
• W2040384050 hasConcept C185592680 @default.
• W2040384050 hasConcept C18705241 @default.
• W2040384050 hasConcept C201194858 @default.
• W2040384050 hasConcept C2776966407 @default.
• W2040384050 hasConcept C2778022156 @default.
• W2040384050 hasConcept C32909587 @default.
• W2040384050 hasConcept C47701112 @default.
• W2040384050 hasConcept C501734568 @default.
• W2040384050 hasConcept C55493867 @default.
• W2040384050 hasConcept C59593255 @default.
• W2040384050 hasConcept C71240020 @default.
• W2040384050 hasConcept C8010536 @default.
• W2040384050 hasConcept C86803240 @default.
• W2040384050 hasConceptScore W2040384050C104317684 @default.
• W2040384050 hasConceptScore W2040384050C105702510 @default.
• W2040384050 hasConceptScore W2040384050C112887158 @default.
• W2040384050 hasConceptScore W2040384050C115624301 @default.
• W2040384050 hasConceptScore W2040384050C12554922 @default.
• W2040384050 hasConceptScore W2040384050C143065580 @default.
• W2040384050 hasConceptScore W2040384050C147597530 @default.
• W2040384050 hasConceptScore W2040384050C178790620 @default.
• W2040384050 hasConceptScore W2040384050C185592680 @default.
• W2040384050 hasConceptScore W2040384050C18705241 @default.
• W2040384050 hasConceptScore W2040384050C201194858 @default.
• W2040384050 hasConceptScore W2040384050C2776966407 @default.
• W2040384050 hasConceptScore W2040384050C2778022156 @default.
• W2040384050 hasConceptScore W2040384050C32909587 @default.
• W2040384050 hasConceptScore W2040384050C47701112 @default.
• W2040384050 hasConceptScore W2040384050C501734568 @default.
• W2040384050 hasConceptScore W2040384050C55493867 @default.
• W2040384050 hasConceptScore W2040384050C59593255 @default.
• W2040384050 hasConceptScore W2040384050C71240020 @default.
• W2040384050 hasConceptScore W2040384050C8010536 @default.

• next