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• W2040402261 abstract "Following infection of 16-week old specific pathogen-free (SPF) female chickens with an enterotropic variant of infectious bronchitis virus (IBV) strain G, IBV-specific Immunoglobulin G (IgG) and IgA were detected in tears, tracheal washes, oviduct washes, duodenal and caecal contents using class-specific monoclonal antibodies in enzyme linked immunosorbent assays (ELISA). IgG antibody content was highest in tears on Day 7 post-infection (p.i.) and was still detectable on Day 23 p.i. Significant levels of IgG antibody were present in oviduct washes on Days 7 and 23 p.i. Tears showed the highest IgA antibody concentration of any sample on Day 7 p.i. but this decreased to an insignificant level by Day 17 p.i. Oviduct and tracheal washes had IgA antibodies on Day 7 p.i. Significant levels of both antibody classes were detected in duodenal contents on Day 17 p.i. only but neither was present in caecal contents. Using a method which compares titre of antibody produced from pieces of tissue at 4°C with that at 37°C as an indicator of local secretion, IgG and IgA antibody synthesis was demonstrated in the Harderian gland on Days 7 and 17 p.i. Local IgG antibody synthesis was seen in the oviduct on Day 7 p.i. but on Day 23 p.i. the optical density at 4°C was higher than at 37°C, suggestive of high concentrations of extracellular antibody. In a separate experiment, passively administered IBV antibody transuded from serum into oviduct washes but did not do so in the trachea. In a third experiment, ELISA and haemagglutination-inhibition (HI) titres of serum and oviduct washes from ten hens previously infected when day-old were compared with egg production. Maximum positive correlation was seen between HI titres of oviduct washes and egg production." @default.
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• W2040402261 date "1996-09-01" @default.
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• W2040402261 title "Local antibody production in the oviduct and gut of hens infected with a variant strain of infectious bronchitis virus" @default.
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• W2040402261 doi "https://doi.org/10.1016/0165-2427(95)05545-2" @default.
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