FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040415740> ?p ?o ?g. }

• W2040415740 endingPage "15044" @default.
• W2040415740 startingPage "15039" @default.
• W2040415740 abstract "The human epidermal growth factor receptor 2 (HER2) is specifically overexpressed in tumors of several cancers, including an aggressive form of breast cancer. It is therefore a target for both cancer diagnostics and therapy. The 58 amino acid residue Z her 2 affibody molecule was previously engineered as a high-affinity binder of HER2. Here we determined the structure of Z her 2 in solution and the crystal structure of Z her 2 in complex with the HER2 extracellular domain. Z her 2 binds to a conformational epitope on HER2 that is distant from those recognized by the therapeutic antibodies trastuzumab and pertuzumab. Its small size and lack of interference may provide Z her 2 with advantages for diagnostic use or even for delivery of therapeutic agents to HER2-expressing tumors when trastuzumab or pertuzumab are already employed. Biophysical characterization shows that Z her 2 is thermodynamically stable in the folded state yet undergoing conformational interconversion on a submillisecond time scale. The data suggest that it is the HER2-binding conformation that is formed transiently prior to binding. Still, binding is very strong with a dissociation constant K D = 22 pM, and perfect conformational homogeneity is therefore not necessarily required in engineered binding proteins. A comparison of the original Z domain scaffold to free and bound Z her 2 structures reveals how high-affinity binding has evolved during selection and affinity maturation and suggests how a compromise between binding surface optimization and stability and dynamics of the unbound state has been reached." @default.
• W2040415740 created "2016-06-24" @default.
• W2040415740 creator A5005212856 @default.
• W2040415740 creator A5023086957 @default.
• W2040415740 creator A5059271995 @default.
• W2040415740 creator A5063659558 @default.
• W2040415740 creator A5083509237 @default.
• W2040415740 date "2010-08-09" @default.
• W2040415740 modified "2023-10-03" @default.
• W2040415740 title "Structural basis for high-affinity HER2 receptor binding by an engineered protein" @default.
• W2040415740 cites W1563420247 @default.
• W2040415740 cites W1966886639 @default.
• W2040415740 cites W1971267920 @default.
• W2040415740 cites W1971619188 @default.
• W2040415740 cites W1975917154 @default.
• W2040415740 cites W1984007340 @default.
• W2040415740 cites W1985312565 @default.
• W2040415740 cites W1989265777 @default.
• W2040415740 cites W1993870692 @default.
• W2040415740 cites W2002831204 @default.
• W2040415740 cites W2006003458 @default.
• W2040415740 cites W2020372547 @default.
• W2040415740 cites W2024651005 @default.
• W2040415740 cites W2030437687 @default.
• W2040415740 cites W2035758624 @default.
• W2040415740 cites W2051845341 @default.
• W2040415740 cites W2052917877 @default.
• W2040415740 cites W2073728244 @default.
• W2040415740 cites W2084295374 @default.
• W2040415740 cites W2086429702 @default.
• W2040415740 cites W2092897704 @default.
• W2040415740 cites W2103719918 @default.
• W2040415740 cites W2104239861 @default.
• W2040415740 cites W2123206362 @default.
• W2040415740 cites W2131443651 @default.
• W2040415740 cites W2134450909 @default.
• W2040415740 cites W2151654128 @default.
• W2040415740 cites W2160125502 @default.
• W2040415740 cites W2163065611 @default.
• W2040415740 cites W2165003903 @default.
• W2040415740 cites W2166848357 @default.
• W2040415740 cites W2167762619 @default.
• W2040415740 doi "https://doi.org/10.1073/pnas.1005025107" @default.
• W2040415740 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2930565" @default.
• W2040415740 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20696930" @default.
• W2040415740 hasPublicationYear "2010" @default.
• W2040415740 type Work @default.
• W2040415740 sameAs 2040415740 @default.
• W2040415740 citedByCount "171" @default.
• W2040415740 countsByYear W20404157402012 @default.
• W2040415740 countsByYear W20404157402013 @default.
• W2040415740 countsByYear W20404157402014 @default.
• W2040415740 countsByYear W20404157402015 @default.
• W2040415740 countsByYear W20404157402016 @default.
• W2040415740 countsByYear W20404157402017 @default.
• W2040415740 countsByYear W20404157402018 @default.
• W2040415740 countsByYear W20404157402019 @default.
• W2040415740 countsByYear W20404157402020 @default.
• W2040415740 countsByYear W20404157402021 @default.
• W2040415740 countsByYear W20404157402022 @default.
• W2040415740 countsByYear W20404157402023 @default.
• W2040415740 crossrefType "journal-article" @default.
• W2040415740 hasAuthorship W2040415740A5005212856 @default.
• W2040415740 hasAuthorship W2040415740A5023086957 @default.
• W2040415740 hasAuthorship W2040415740A5059271995 @default.
• W2040415740 hasAuthorship W2040415740A5063659558 @default.
• W2040415740 hasAuthorship W2040415740A5083509237 @default.
• W2040415740 hasBestOaLocation W20404157402 @default.
• W2040415740 hasConcept C107824862 @default.
• W2040415740 hasConcept C121608353 @default.
• W2040415740 hasConcept C12554922 @default.
• W2040415740 hasConcept C147816474 @default.
• W2040415740 hasConcept C159654299 @default.
• W2040415740 hasConcept C170493617 @default.
• W2040415740 hasConcept C181199279 @default.
• W2040415740 hasConcept C185592680 @default.
• W2040415740 hasConcept C195616568 @default.
• W2040415740 hasConcept C203014093 @default.
• W2040415740 hasConcept C2777339483 @default.
• W2040415740 hasConcept C2779786085 @default.
• W2040415740 hasConcept C2781164504 @default.
• W2040415740 hasConcept C47701112 @default.
• W2040415740 hasConcept C51639874 @default.
• W2040415740 hasConcept C530470458 @default.
• W2040415740 hasConcept C54355233 @default.
• W2040415740 hasConcept C55493867 @default.
• W2040415740 hasConcept C71240020 @default.
• W2040415740 hasConcept C74998103 @default.
• W2040415740 hasConcept C86803240 @default.
• W2040415740 hasConceptScore W2040415740C107824862 @default.
• W2040415740 hasConceptScore W2040415740C121608353 @default.
• W2040415740 hasConceptScore W2040415740C12554922 @default.
• W2040415740 hasConceptScore W2040415740C147816474 @default.
• W2040415740 hasConceptScore W2040415740C159654299 @default.
• W2040415740 hasConceptScore W2040415740C170493617 @default.
• W2040415740 hasConceptScore W2040415740C181199279 @default.
• W2040415740 hasConceptScore W2040415740C185592680 @default.
• W2040415740 hasConceptScore W2040415740C195616568 @default.

• next