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- W2040440130 abstract "Recombinant ricin A chain was irreversibly modified by Procion blue MX-R, a dichlorotriazinyl analogue of Cibacron blue F3G-A, at pH 7.5 and 4 degrees C in 90 h with over 95% loss of activity in an in vitro translation assay. The presence of total yeast RNA reduced the covalent attachment of Procion blue MX-R to ricin A chain. Quantitatively modified ricin A chain contained 2 mol Procion blue MX-R/mol 29-kDa subunit. Tryptic digestion and resolution of the peptides by reverse-phase high-performance liquid chromatography yields a blue peptide corresponding to Gln5-Arg26 of ricin A chain. Thus, a likely dye-binding site on recombinant ricin A was identified. This region is removed from the active-site cleft of recombinant ricin A but may be involved in its substrate binding." @default.
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- W2040440130 date "1995-11-01" @default.
- W2040440130 modified "2023-10-17" @default.
- W2040440130 title "Affinity Labeling of Recombinant Ricin A Chain with Procion Blue MX-R" @default.
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- W2040440130 doi "https://doi.org/10.1111/j.1432-1033.1995.880_3.x" @default.
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