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- W2040452495 abstract "Postcapillary affinity detection is developed to analyze structurally related compounds following capillary zone electrophoresis separation. In this work, biotin and biotin derivatives are resolved by capillary zone electrophoresis and selectively identified on the basis of their cross-reactivity with streptavidin−fluorescein isothiocyanate, added via a postcapillary reactor. Direct sensing of the affinity reaction is achieved by monitoring the resulting fluorescence enhancement of streptavidin−fluorescein isothiocyanate upon biotin binding. The effects of postcapillary reactor parameters, including capillary gap distance, dilution ratio, and reaction distance, on peak area and peak efficiency of the biotin−streptavidin complex are investigated. A reaction kinetic model is presented for evaluating the dynamic range and detection limits of postcapillary affinity detection as a function of streptavidin concentration in the postcapillary reactor. The detection limit for postcapillary affinity detection of biotin is on the order of 3 nM, or 4 fg." @default.
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- W2040452495 date "1997-12-01" @default.
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- W2040452495 title "Mechanistic Studies of Postcapillary Affinity Detection for Capillary Zone Electrophoresis Based on the Biotin−Streptavidin System" @default.
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- W2040452495 doi "https://doi.org/10.1021/ac9705879" @default.
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