FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040488285> ?p ?o ?g. }

• W2040488285 endingPage "9189" @default.
• W2040488285 startingPage "9174" @default.
• W2040488285 abstract "ABSTRACT DNA vaccines have been successful in eliciting potent immune responses in mice. Their efficiency, however, is restricted in larger animals. One reason for the limited performance of the DNA vaccines is the lack of molecular strategies to enhance immune responses. Additionally, genes directly cloned from pathogenic organisms may not be efficiently translated in a heterologous host expression system as a consequence of codon bias. To evaluate the influence of codon optimization on the immune response, we elected to use the Tat antigens of human immunodeficiency virus type 1 (HIV-1) (subtype C) and HIV-2, as these viral antigens are poorly immunogenic in natural infection and in experimental immunization and they are functionally important in viral infectivity and pathogenesis. Substituting codons that are optimally used in the mammalian system, we synthetically assembled Tat genes and compared them with the wild-type counterparts in two different mouse strains. Codon-optimized Tat genes induced qualitatively and quantitatively superior immune responses as measured in a T-cell proliferation assay, enzyme-linked immunospot assay, and chromium release assay. Importantly, while the wild-type genes promoted a mixed Th1-Th2-type cytokine profile, the codon-optimized genes induced a predominantly Th1 profile. Using a pepscan strategy, we mapped an immunodominant T-helper epitope to the core and basic domains of HIV-1 Tat. We also identified cross-clade immune responses between HIV-1 subtype B and C Tat proteins mapped to this T-helper epitope. Developing molecular strategies to optimize the immunogenicity of DNA vaccines is critical for inducing strong immune responses, especially to antigens like Tat. Our identification of a highly conserved T-helper epitope in the first exon of HIV-1 Tat of subtype C and the demonstration of a cross-clade immune response between subtypes B and C are important for a more rational design of an HIV vaccine." @default.
• W2040488285 created "2016-06-24" @default.
• W2040488285 creator A5003417272 @default.
• W2040488285 creator A5020997464 @default.
• W2040488285 creator A5041672067 @default.
• W2040488285 creator A5051560305 @default.
• W2040488285 date "2004-09-01" @default.
• W2040488285 modified "2023-09-26" @default.
• W2040488285 title "Codon Optimization of the Tat Antigen of Human Immunodeficiency Virus Type 1 Generates Strong Immune Responses in Mice following Genetic Immunization" @default.
• W2040488285 cites W135947367 @default.
• W2040488285 cites W1481002459 @default.
• W2040488285 cites W1566505170 @default.
• W2040488285 cites W1594844469 @default.
• W2040488285 cites W1604626968 @default.
• W2040488285 cites W1605749243 @default.
• W2040488285 cites W1633472206 @default.
• W2040488285 cites W1669155652 @default.
• W2040488285 cites W1679796753 @default.
• W2040488285 cites W1813961829 @default.
• W2040488285 cites W1858509526 @default.
• W2040488285 cites W1895622651 @default.
• W2040488285 cites W192763546 @default.
• W2040488285 cites W1927674534 @default.
• W2040488285 cites W1948199170 @default.
• W2040488285 cites W1954929088 @default.
• W2040488285 cites W1963902916 @default.
• W2040488285 cites W1964770087 @default.
• W2040488285 cites W1966700067 @default.
• W2040488285 cites W1967034367 @default.
• W2040488285 cites W1968217509 @default.
• W2040488285 cites W1971592398 @default.
• W2040488285 cites W1973675133 @default.
• W2040488285 cites W1975957969 @default.
• W2040488285 cites W1977775509 @default.
• W2040488285 cites W1978407444 @default.
• W2040488285 cites W1979340502 @default.
• W2040488285 cites W1980203367 @default.
• W2040488285 cites W1981680523 @default.
• W2040488285 cites W1981747163 @default.
• W2040488285 cites W1987914887 @default.
• W2040488285 cites W1990567434 @default.
• W2040488285 cites W1991799944 @default.
• W2040488285 cites W1993977523 @default.
• W2040488285 cites W1996288593 @default.
• W2040488285 cites W1996600503 @default.
• W2040488285 cites W1998670002 @default.
• W2040488285 cites W2006405806 @default.
• W2040488285 cites W2009361016 @default.
• W2040488285 cites W2012889067 @default.
• W2040488285 cites W2014027204 @default.
• W2040488285 cites W2014311539 @default.
• W2040488285 cites W2017821020 @default.
• W2040488285 cites W2021987545 @default.
• W2040488285 cites W2024173220 @default.
• W2040488285 cites W2025062363 @default.
• W2040488285 cites W2028725654 @default.
• W2040488285 cites W2033650683 @default.
• W2040488285 cites W2038665432 @default.
• W2040488285 cites W2039420133 @default.
• W2040488285 cites W2040267802 @default.
• W2040488285 cites W2041110737 @default.
• W2040488285 cites W2046027100 @default.
• W2040488285 cites W2047463213 @default.
• W2040488285 cites W2057123011 @default.
• W2040488285 cites W2060308187 @default.
• W2040488285 cites W2061009669 @default.
• W2040488285 cites W2062348686 @default.
• W2040488285 cites W2064914230 @default.
• W2040488285 cites W2067592236 @default.
• W2040488285 cites W2068397982 @default.
• W2040488285 cites W2075368100 @default.
• W2040488285 cites W2080515993 @default.
• W2040488285 cites W2081326862 @default.
• W2040488285 cites W2081991691 @default.
• W2040488285 cites W2084229810 @default.
• W2040488285 cites W2087395692 @default.
• W2040488285 cites W2087772385 @default.
• W2040488285 cites W2092326634 @default.
• W2040488285 cites W2097755672 @default.
• W2040488285 cites W2101702353 @default.
• W2040488285 cites W2104892940 @default.
• W2040488285 cites W2107755945 @default.
• W2040488285 cites W2107786521 @default.
• W2040488285 cites W2108463233 @default.
• W2040488285 cites W2109347706 @default.
• W2040488285 cites W2109352986 @default.
• W2040488285 cites W2110324093 @default.
• W2040488285 cites W2115620928 @default.
• W2040488285 cites W2115696185 @default.
• W2040488285 cites W2116371336 @default.
• W2040488285 cites W2117751118 @default.
• W2040488285 cites W2119431042 @default.
• W2040488285 cites W2130786792 @default.
• W2040488285 cites W2135884205 @default.
• W2040488285 cites W2138721124 @default.
• W2040488285 cites W2141028568 @default.
• W2040488285 cites W2149791183 @default.
• W2040488285 cites W2151181830 @default.

• next