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- W2040492756 abstract "The optically active urea compounds R- and S-1-α-methylbenzyl-3-p-tolylurea (R- and S-MBTU) have qualitatively different effects on plant physiology, especially in Gramineae plants. To elucidate the mechanisms by which S-MBTU inhibits root growth of wheat (Triticum aestivum L. var. Chinese Spring), we used PCR-based suppression subtractive hybridization (SSH) and quantitative real-time RT-PCR (qPCR) analyses. Genes related to amino acid biosynthesis, cell cycle and skeleton, protein synthesis, cell wall biosynthesis, glycolysis, signaling, DNA modification, and detoxification were enantioselectively regulated. These expression profiles suggested that the putative mode of action of S-MBTU is disruption of primary amino acid biosynthesis, especially the glutamine synthetase (GS)- and acetohydroxyacid synthase (AHAS)-enzyme systems, and disruption of cell membrane skeleton homeostasis related to a putative α-tubulin 2 (α-TUB2), a putative β-actin (ACT) and β-tubulin 1 (β-TUB1). Other genes specifically affected by S-MBTU may result in disruption of metabolic processes, leading to inhibition of root growth." @default.
- W2040492756 created "2016-06-24" @default.
- W2040492756 creator A5037798056 @default.
- W2040492756 creator A5050892744 @default.
- W2040492756 creator A5057356245 @default.
- W2040492756 creator A5073549316 @default.
- W2040492756 date "2010-11-01" @default.
- W2040492756 modified "2023-09-24" @default.
- W2040492756 title "PCR-based suppression subtractive hybridization analyses of enantioselective gene expression in root tips of wheat treated with optically active urea compounds" @default.
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- W2040492756 doi "https://doi.org/10.1016/j.pestbp.2010.08.001" @default.
- W2040492756 hasPublicationYear "2010" @default.
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