FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040497781> ?p ?o ?g. }

• W2040497781 endingPage "187" @default.
• W2040497781 startingPage "177" @default.
• W2040497781 abstract "Vancomycin-resistant enterococci are pathogenic bacteria that attenuate antibiotic sensitivity by producing peptidoglycan precursors that terminate in D-Ala-D-lactate rather than D-Ala-D-Ala. A key enzyme in effecting antibiotic resistance is the metallodipeptidase VanX, which reduces the cellular pool of the D-Ala-D-Ala dipeptide.We constructed eleven mutants, using the recently determined VanX structure as a basis, to investigate residue function. Mutating Asp142 or Ser114 showed a large effect principally on KM, consistent with roles in recognition of the D-Ala-D-Ala termini. The drastic reduction or absence of activity in the Arg71 mutants correlates with a role in the stabilization of an anionic tetrahedral transition state. Three residues of the Escherichia coli D-Ala-D-Ala ligase (Ddl), Glu15, Ser 281 and Arg255, are similarly conserved and have equivalent functions with respect to VanX, consistent with a convergent evolution of active sites to bind D-Ala-D-Ala and lower energy barriers for formation of the tetrahedral intermediate and transition states. In the N-acyl-D-Ala-D-Ala carboxypeptidase VanY, all active-site residues are conserved (except for the two responsible for recognition of the dipeptide amino terminus).The mutagenesis results support structure-based functional predictions and explain why the VanX dipeptidase and Ddl ligase show narrow specificity for the D,D-dipeptide substrate. The results reveal that VanX and Ddl, two enzymes that use the same substrate but proceed in opposite directions driven by distinct cofactors (zinc versus ATP), evolved similar architectural solutions to substrate recognition and catalysis acceleration. VanY sequence analysis predicts an active site and mechanism of reaction similar to VanX." @default.
• W2040497781 created "2016-06-24" @default.
• W2040497781 creator A5020735084 @default.
• W2040497781 creator A5060579310 @default.
• W2040497781 date "1999-03-01" @default.
• W2040497781 modified "2023-09-26" @default.
• W2040497781 title "Mutational analysis of active-site residues of the enterococcal d-Ala-d-Ala dipeptidase VanX and comparison with Escherichia colid-Ala-d-Ala ligase and d-Ala-d-Ala carboxypeptidase VanY" @default.
• W2040497781 cites W1563365395 @default.
• W2040497781 cites W1628177336 @default.
• W2040497781 cites W1640953885 @default.
• W2040497781 cites W1924960875 @default.
• W2040497781 cites W1969292393 @default.
• W2040497781 cites W1969648984 @default.
• W2040497781 cites W1980595924 @default.
• W2040497781 cites W1985107413 @default.
• W2040497781 cites W1986894327 @default.
• W2040497781 cites W1992184302 @default.
• W2040497781 cites W1994340528 @default.
• W2040497781 cites W1995412539 @default.
• W2040497781 cites W1999545809 @default.
• W2040497781 cites W2019702615 @default.
• W2040497781 cites W2026407337 @default.
• W2040497781 cites W2032118018 @default.
• W2040497781 cites W2035889721 @default.
• W2040497781 cites W2039634842 @default.
• W2040497781 cites W2040095685 @default.
• W2040497781 cites W2050717506 @default.
• W2040497781 cites W2060913542 @default.
• W2040497781 cites W2074968287 @default.
• W2040497781 cites W2077968226 @default.
• W2040497781 cites W2081294871 @default.
• W2040497781 cites W2093482351 @default.
• W2040497781 cites W2100837269 @default.
• W2040497781 cites W2104017463 @default.
• W2040497781 cites W2106315897 @default.
• W2040497781 cites W2106882534 @default.
• W2040497781 cites W2114519194 @default.
• W2040497781 cites W2119262069 @default.
• W2040497781 cites W2119586900 @default.
• W2040497781 cites W2129263647 @default.
• W2040497781 cites W2134460557 @default.
• W2040497781 cites W2137502578 @default.
• W2040497781 cites W2153668434 @default.
• W2040497781 cites W2332177486 @default.
• W2040497781 cites W4243643148 @default.
• W2040497781 cites W4249231186 @default.
• W2040497781 cites W4253547259 @default.
• W2040497781 doi "https://doi.org/10.1016/s1074-5521(99)89009-7" @default.
• W2040497781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10074467" @default.
• W2040497781 hasPublicationYear "1999" @default.
• W2040497781 type Work @default.
• W2040497781 sameAs 2040497781 @default.
• W2040497781 citedByCount "35" @default.
• W2040497781 countsByYear W20404977812012 @default.
• W2040497781 countsByYear W20404977812013 @default.
• W2040497781 countsByYear W20404977812014 @default.
• W2040497781 countsByYear W20404977812015 @default.
• W2040497781 countsByYear W20404977812019 @default.
• W2040497781 crossrefType "journal-article" @default.
• W2040497781 hasAuthorship W2040497781A5020735084 @default.
• W2040497781 hasAuthorship W2040497781A5060579310 @default.
• W2040497781 hasBestOaLocation W20404977811 @default.
• W2040497781 hasConcept C104317684 @default.
• W2040497781 hasConcept C143065580 @default.
• W2040497781 hasConcept C175114707 @default.
• W2040497781 hasConcept C181199279 @default.
• W2040497781 hasConcept C2776634448 @default.
• W2040497781 hasConcept C2779138802 @default.
• W2040497781 hasConcept C2779281246 @default.
• W2040497781 hasConcept C41183919 @default.
• W2040497781 hasConcept C547475151 @default.
• W2040497781 hasConcept C55493867 @default.
• W2040497781 hasConcept C80458899 @default.
• W2040497781 hasConcept C86803240 @default.
• W2040497781 hasConceptScore W2040497781C104317684 @default.
• W2040497781 hasConceptScore W2040497781C143065580 @default.
• W2040497781 hasConceptScore W2040497781C175114707 @default.
• W2040497781 hasConceptScore W2040497781C181199279 @default.
• W2040497781 hasConceptScore W2040497781C2776634448 @default.
• W2040497781 hasConceptScore W2040497781C2779138802 @default.
• W2040497781 hasConceptScore W2040497781C2779281246 @default.
• W2040497781 hasConceptScore W2040497781C41183919 @default.
• W2040497781 hasConceptScore W2040497781C547475151 @default.
• W2040497781 hasConceptScore W2040497781C55493867 @default.
• W2040497781 hasConceptScore W2040497781C80458899 @default.
• W2040497781 hasConceptScore W2040497781C86803240 @default.
• W2040497781 hasIssue "3" @default.
• W2040497781 hasLocation W20404977811 @default.
• W2040497781 hasLocation W20404977812 @default.
• W2040497781 hasOpenAccess W2040497781 @default.
• W2040497781 hasPrimaryLocation W20404977811 @default.
• W2040497781 hasRelatedWork W153595000 @default.
• W2040497781 hasRelatedWork W1608463667 @default.
• W2040497781 hasRelatedWork W2006724716 @default.
• W2040497781 hasRelatedWork W2029350330 @default.
• W2040497781 hasRelatedWork W2042417706 @default.
• W2040497781 hasRelatedWork W2096895823 @default.
• W2040497781 hasRelatedWork W2119573275 @default.

• next