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- W2040497793 abstract "To study the effect of sperm concentration on the results of pig in vitro fertilization (IVF), 313 oocytes recovered from oviducts of prepubertal gilts after induction of ovulation were used. After capacitation, the number of live spermatozoa in the fertilization dishes was adjusted to 3 × 105, 6 × 105 and 12 × 105 cell/ml. After 4 hours of co-culture in TCM-199, the oocytes were pippeted to remove cumulus cells and the excess spermatozoa around the zona pellucida, and were transferred to fresh TCM-199 for another 1214hours. The results showed that 6 × 105 spermatozoa/ml is the optimum concentration for this system; the percentage of fertilized ova (71.6%) was not different from the best (76.8%), that was obtained with the highest concentration, and the percentage of monospermy (62.3%) was not different from the best (68.1%), that was obtained with the lowest concentration. The percentage of spermatozoa that reached the pronuclear stage increased while sperm concentration was decreased. The percentage of spermatozoa at the decondensed stage was decreased when the sperm concentration increased." @default.
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- W2040497793 date "1993-09-01" @default.
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- W2040497793 title "Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs" @default.
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- W2040497793 doi "https://doi.org/10.1016/0093-691x(93)90407-v" @default.
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