Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040542401> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2040542401 endingPage "231" @default.
- W2040542401 startingPage "224" @default.
- W2040542401 abstract "We have studied the sequence requirements for 3'-end formation of rDNA transcripts in a cell-free system and show that the generation of correct ends of mouse pre-rRNA is brought about by a two-step process that involves a bona fide termination reaction, followed by a specific trimming of the primary transcript by 10 nucleotides. We show that termination of mouse ribosomal gene transcription by RNA polymerase I (pol I) takes place in front of an 18-bp DNA sequence element (the 'Sal box'), which was previously shown to function as termination signal. Termination of pol I transcription occurs at a fixed distance (11 bp) upstream of the Sal box, independent of the sequence of adjacent gene regions. The processing reaction, however, is strongly influenced by sequences flanking the termination signal at the 5' site. Substitution of a cluster of T residues by guanines within the region of 3'-end formation abolishes the 3'-terminal trimming of the primary transcript. Interestingly, this 3'-terminal processing event, which can be uncoupled from the termination reaction, requires both a correct 3' end and specific sequences in the 3'-terminal region of the primary transcript. Read-through transcripts generated in the extract system or by SP6 RNA polymerase are no substrate for the processing nuclease(s). Because the termination and processing activity can be separated chromatographically, the nucleolytic activity does not reside in TTF-I, the factor that binds to the Sal box and directs transcription termination." @default.
- W2040542401 created "2016-06-24" @default.
- W2040542401 creator A5005683105 @default.
- W2040542401 creator A5060467982 @default.
- W2040542401 date "1989-02-01" @default.
- W2040542401 modified "2023-09-25" @default.
- W2040542401 title "3'-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction." @default.
- W2040542401 cites W1535907141 @default.
- W2040542401 cites W163264966 @default.
- W2040542401 cites W1965372133 @default.
- W2040542401 cites W1967274237 @default.
- W2040542401 cites W1975642325 @default.
- W2040542401 cites W1993243592 @default.
- W2040542401 cites W2001020273 @default.
- W2040542401 cites W2016605966 @default.
- W2040542401 cites W2020070631 @default.
- W2040542401 cites W2024651141 @default.
- W2040542401 cites W2059112193 @default.
- W2040542401 cites W2060333964 @default.
- W2040542401 cites W2073999982 @default.
- W2040542401 cites W2087844645 @default.
- W2040542401 cites W2094778829 @default.
- W2040542401 cites W2109354206 @default.
- W2040542401 cites W2143488845 @default.
- W2040542401 cites W2144471538 @default.
- W2040542401 cites W2147800702 @default.
- W2040542401 cites W2940846803 @default.
- W2040542401 cites W91137656 @default.
- W2040542401 doi "https://doi.org/10.1101/gad.3.2.224" @default.
- W2040542401 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2714650" @default.
- W2040542401 hasPublicationYear "1989" @default.
- W2040542401 type Work @default.
- W2040542401 sameAs 2040542401 @default.
- W2040542401 citedByCount "67" @default.
- W2040542401 countsByYear W20405424012012 @default.
- W2040542401 countsByYear W20405424012013 @default.
- W2040542401 countsByYear W20405424012017 @default.
- W2040542401 countsByYear W20405424012019 @default.
- W2040542401 countsByYear W20405424012021 @default.
- W2040542401 crossrefType "journal-article" @default.
- W2040542401 hasAuthorship W2040542401A5005683105 @default.
- W2040542401 hasAuthorship W2040542401A5060467982 @default.
- W2040542401 hasBestOaLocation W20405424011 @default.
- W2040542401 hasConcept C101762097 @default.
- W2040542401 hasConcept C104317684 @default.
- W2040542401 hasConcept C138885662 @default.
- W2040542401 hasConcept C150194340 @default.
- W2040542401 hasConcept C153911025 @default.
- W2040542401 hasConcept C179926584 @default.
- W2040542401 hasConcept C2776449523 @default.
- W2040542401 hasConcept C41895202 @default.
- W2040542401 hasConcept C51807208 @default.
- W2040542401 hasConcept C54355233 @default.
- W2040542401 hasConcept C54458228 @default.
- W2040542401 hasConcept C64350747 @default.
- W2040542401 hasConcept C67585878 @default.
- W2040542401 hasConcept C67705224 @default.
- W2040542401 hasConcept C86803240 @default.
- W2040542401 hasConcept C95444343 @default.
- W2040542401 hasConceptScore W2040542401C101762097 @default.
- W2040542401 hasConceptScore W2040542401C104317684 @default.
- W2040542401 hasConceptScore W2040542401C138885662 @default.
- W2040542401 hasConceptScore W2040542401C150194340 @default.
- W2040542401 hasConceptScore W2040542401C153911025 @default.
- W2040542401 hasConceptScore W2040542401C179926584 @default.
- W2040542401 hasConceptScore W2040542401C2776449523 @default.
- W2040542401 hasConceptScore W2040542401C41895202 @default.
- W2040542401 hasConceptScore W2040542401C51807208 @default.
- W2040542401 hasConceptScore W2040542401C54355233 @default.
- W2040542401 hasConceptScore W2040542401C54458228 @default.
- W2040542401 hasConceptScore W2040542401C64350747 @default.
- W2040542401 hasConceptScore W2040542401C67585878 @default.
- W2040542401 hasConceptScore W2040542401C67705224 @default.
- W2040542401 hasConceptScore W2040542401C86803240 @default.
- W2040542401 hasConceptScore W2040542401C95444343 @default.
- W2040542401 hasIssue "2" @default.
- W2040542401 hasLocation W20405424011 @default.
- W2040542401 hasLocation W20405424012 @default.
- W2040542401 hasOpenAccess W2040542401 @default.
- W2040542401 hasPrimaryLocation W20405424011 @default.
- W2040542401 hasRelatedWork W1555221542 @default.
- W2040542401 hasRelatedWork W1983021027 @default.
- W2040542401 hasRelatedWork W2002184841 @default.
- W2040542401 hasRelatedWork W2008301391 @default.
- W2040542401 hasRelatedWork W2040542401 @default.
- W2040542401 hasRelatedWork W2063343276 @default.
- W2040542401 hasRelatedWork W2075341922 @default.
- W2040542401 hasRelatedWork W2108624464 @default.
- W2040542401 hasRelatedWork W2142889112 @default.
- W2040542401 hasRelatedWork W4246919179 @default.
- W2040542401 hasVolume "3" @default.
- W2040542401 isParatext "false" @default.
- W2040542401 isRetracted "false" @default.
- W2040542401 magId "2040542401" @default.
- W2040542401 workType "article" @default.