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- W2040573039 abstract "A novel high-performance liquid chromatographic method was developded for the determination in plasma samples of the synthetic octapeptide SK&F 105494 {O-ethyl-d-tyrosyl-l-phenylalanyl-l-asparaginyl-5-[1-(carboxymethyl)cyclohexyl]-l-norvalyl-l-arginyl-d-arginamide, cyclic (5–1) peptide}, and active aquaretic agent which significantly increases water excretion in experimental animal models through competitive antagonism of renal epithelial vasopressin receptors. The method involves isolation of Sk&F 105494 from plasma samples by solid phase extraction prior to chromatogrpahic analysis. Following chromatographic separation, in-line fluorescence detection was accomplished via a selective-post-column reaction of the guanidino group of the arginine moiety of the peptide withalkaline ninhydrin to generate a highly fluorescent product. Optimization of the post-column reaction conditions and the use of high-performance liquid chromatography columns of reduced internal diameter (2.1 mm), resulted in an on-column detection limit of 50pg (45 fmol). The limit of sensitivity in plasma was 0.5 ng/ml. The assay was linear over the range 0.5–100 ng/ml. Precision and accuracy were within 11% across the calibration range. The assay was shown to be suitable to study the pharmacokinetics of SK&F 105494 in human subjects and animals. In addition, the methodology developed has general applicability in the detection of arginine-containing peptides in biololgical matrices." @default.
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- W2040573039 title "High-performance liquid chromatographic determination of an arginine-containing octapeptide antagonist of vasopressin in human plasma by means of a selective post-column reaction with fluorescence detection" @default.
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- W2040573039 doi "https://doi.org/10.1016/s0021-9673(01)84183-2" @default.
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