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• W2040586980 abstract "Lactococcus lactis UQ2 is a wild nisin A producer isolated from a Mexican cheese that grows poorly in milk. Conjugal matings with L. lactis NCDO712 to transfer the Lac+ Prt+ plasmid pLP712 and selection with nisin and lactose yielded L. lactis NCDO712 NisA+. Naturally rifampicin resistant L. lactis UQ2Rif was isolated to provide an additional selective marker. The identity of a transconjugant L. lactis UQ2Rif Lac+ was confirmed by RAPD-PCR fingerprinting, nisA PCR amplification, nisin production, presence of pLP712 and phospho-β-galactosidase activity. This strain performed well in milk and synthesised 200 IU/mL nisin, 40 times more than the original strain." @default.
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• W2040586980 date "2010-07-06" @default.
• W2040586980 modified "2023-09-24" @default.
• W2040586980 title "Enhancement of nisin production in milk by conjugal transfer of the protease-lactose plasmid pLP712 to the wild strain Lactococcus lactis UQ2" @default.
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• W2040586980 doi "https://doi.org/10.1111/j.1471-0307.2010.00618.x" @default.
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