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- W2040596113 abstract "ESCRT components function to form multivesicular bodies for sorting of proteins destined to the yeast vacuole. The calcium hypersensitivity of ESCRT mutants is mainly due to repressed expression of PMR1 through the Rim101/Nrg1 pathway in budding yeast. Here, we show that overexpression of PMC1 and its negative regulator gene NYV1 suppresses and increases calcium hypersensitivity of ESCRT mutants, respectively. Consistently, deletion of NYV1 suppresses their calcium hypersensitivity. Expression of NYV1 is dramatically reduced in ESCRT mutants. Promoter analysis demonstrates that both Nrg1 and Mig1 repress NYV1 expression. Deletion of ESCRTs increases Nrg1 binding, but not Mig1‐binding, to the NYV1 promoter. Deletion of MIG1 increases calcium sensitivity of ESCRT mutants due to derepression of NYV1 expression." @default.
- W2040596113 created "2016-06-24" @default.
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- W2040596113 date "2014-07-10" @default.
- W2040596113 modified "2023-10-17" @default.
- W2040596113 title "Expression of <i>NYV1</i> encoding the negative regulator of Pmc1 is repressed by two transcriptional repressors, Nrg1 and Mig1" @default.
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- W2040596113 doi "https://doi.org/10.1016/j.febslet.2014.06.062" @default.
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