FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040605613> ?p ?o ?g. }

• W2040605613 endingPage "731" @default.
• W2040605613 startingPage "722" @default.
• W2040605613 abstract "Cell fate determination between self-renewal or differentiation of spermatogonial stem cells (SSCs) in the testis is precisely regulated to maintain normal spermatogenesis. However, the mechanisms underlying the process remain elusive. To address the problem, we developed a model SSC culture system, first, by establishing techniques to obtain enriched populations of stem cells, and second, by establishing a serum-free culture medium. Flow cytometric cell sorting and the SSC transplantation assay demonstrated that Thy-1 is a unique surface marker of SSCs in neonatal, pup, and adult testes of the mouse. Although the surface phenotype of SSCs is major histocompatibility complex class I(-) Thy-1(+) alpha 6-integrin(+) alpha v-integrin(-/dim) throughout postnatal life, the most enriched population of SSCs was obtained from cryptorchid adult testes by cell-sorting techniques based on Thy-1 expression. This enriched population of SSCs was used to develop a culture system that consisted of serum-free defined medium and STO (SIM mouse embryo-derived thioguanine and ouabain resistant) feeders, which routinely maintained stem cell activity for 1 wk. Combining the culture system and the transplantation assay provided a mechanism to study the effect of single growth factors. A negative effect was demonstrated for several concentrations of basic fibroblast growth factor and leukemia inhibitory factor, whereas glial cell line-derived neurotrophic factor and stem cell factor appeared to have a positive effect on stem cell maintenance. The stem cell enrichment strategies and the culture methods described provide a reproducible and powerful assay system to establish the effect of various environmental factors on SSC survival and replication in vitro." @default.
• W2040605613 created "2016-06-24" @default.
• W2040605613 creator A5010867955 @default.
• W2040605613 creator A5076284560 @default.
• W2040605613 creator A5089013559 @default.
• W2040605613 date "2004-09-01" @default.
• W2040605613 modified "2023-10-17" @default.
• W2040605613 title "Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse Spermatogonial Stem Cells1" @default.
• W2040605613 cites W1489352447 @default.
• W2040605613 cites W1505671001 @default.
• W2040605613 cites W1516316082 @default.
• W2040605613 cites W151805421 @default.
• W2040605613 cites W1703256186 @default.
• W2040605613 cites W1963940266 @default.
• W2040605613 cites W1973035870 @default.
• W2040605613 cites W1976183817 @default.
• W2040605613 cites W1992160731 @default.
• W2040605613 cites W1993491850 @default.
• W2040605613 cites W1997218268 @default.
• W2040605613 cites W2001696564 @default.
• W2040605613 cites W2002654571 @default.
• W2040605613 cites W2008661167 @default.
• W2040605613 cites W2016789158 @default.
• W2040605613 cites W2018855387 @default.
• W2040605613 cites W2020234109 @default.
• W2040605613 cites W2021094253 @default.
• W2040605613 cites W2021971921 @default.
• W2040605613 cites W2025705782 @default.
• W2040605613 cites W2044423229 @default.
• W2040605613 cites W2045847970 @default.
• W2040605613 cites W2046442503 @default.
• W2040605613 cites W2053997148 @default.
• W2040605613 cites W2059033739 @default.
• W2040605613 cites W2062867597 @default.
• W2040605613 cites W2065737043 @default.
• W2040605613 cites W2065914616 @default.
• W2040605613 cites W2067924039 @default.
• W2040605613 cites W2076204859 @default.
• W2040605613 cites W2088227793 @default.
• W2040605613 cites W2094557730 @default.
• W2040605613 cites W2109790916 @default.
• W2040605613 cites W2121877384 @default.
• W2040605613 cites W2131238404 @default.
• W2040605613 cites W2144134929 @default.
• W2040605613 cites W2144166281 @default.
• W2040605613 cites W2153416167 @default.
• W2040605613 cites W2158434412 @default.
• W2040605613 cites W2159999899 @default.
• W2040605613 cites W2165892496 @default.
• W2040605613 cites W2166384143 @default.
• W2040605613 cites W2170748214 @default.
• W2040605613 cites W2260001467 @default.
• W2040605613 doi "https://doi.org/10.1095/biolreprod.104.029207" @default.
• W2040605613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15115718" @default.
• W2040605613 hasPublicationYear "2004" @default.
• W2040605613 type Work @default.
• W2040605613 sameAs 2040605613 @default.
• W2040605613 citedByCount "319" @default.
• W2040605613 countsByYear W20406056132012 @default.
• W2040605613 countsByYear W20406056132013 @default.
• W2040605613 countsByYear W20406056132014 @default.
• W2040605613 countsByYear W20406056132015 @default.
• W2040605613 countsByYear W20406056132016 @default.
• W2040605613 countsByYear W20406056132017 @default.
• W2040605613 countsByYear W20406056132018 @default.
• W2040605613 countsByYear W20406056132019 @default.
• W2040605613 countsByYear W20406056132020 @default.
• W2040605613 countsByYear W20406056132021 @default.
• W2040605613 countsByYear W20406056132022 @default.
• W2040605613 countsByYear W20406056132023 @default.
• W2040605613 crossrefType "journal-article" @default.
• W2040605613 hasAuthorship W2040605613A5010867955 @default.
• W2040605613 hasAuthorship W2040605613A5076284560 @default.
• W2040605613 hasAuthorship W2040605613A5089013559 @default.
• W2040605613 hasBestOaLocation W20406056131 @default.
• W2040605613 hasConcept C104317684 @default.
• W2040605613 hasConcept C126322002 @default.
• W2040605613 hasConcept C144024400 @default.
• W2040605613 hasConcept C145103041 @default.
• W2040605613 hasConcept C149923435 @default.
• W2040605613 hasConcept C16685009 @default.
• W2040605613 hasConcept C170493617 @default.
• W2040605613 hasConcept C203014093 @default.
• W2040605613 hasConcept C27284151 @default.
• W2040605613 hasConcept C2775960820 @default.
• W2040605613 hasConcept C2777587049 @default.
• W2040605613 hasConcept C2778630268 @default.
• W2040605613 hasConcept C28328180 @default.
• W2040605613 hasConcept C2908647359 @default.
• W2040605613 hasConcept C2911091166 @default.
• W2040605613 hasConcept C54355233 @default.
• W2040605613 hasConcept C553184892 @default.
• W2040605613 hasConcept C71924100 @default.
• W2040605613 hasConcept C86803240 @default.
• W2040605613 hasConcept C95444343 @default.
• W2040605613 hasConceptScore W2040605613C104317684 @default.
• W2040605613 hasConceptScore W2040605613C126322002 @default.
• W2040605613 hasConceptScore W2040605613C144024400 @default.

• next