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• W2040618577 abstract "Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens ( n = 8)." @default.
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• W2040618577 date "2005-08-01" @default.
• W2040618577 modified "2023-10-01" @default.
• W2040618577 title "Retroviral expression screening of oncogenes in natural killer cell leukemia" @default.
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• W2040618577 doi "https://doi.org/10.1016/j.leukres.2005.02.002" @default.
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