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- W2040641033 abstract "Placental Glucose Transporter (GLUT1) Expression in Pre-Eclampsia. INTRODUCTION: Glucose is the most important substrate for fetalgrowth. Indeed, there is no significant de novo glucose synthesis inthe fetus and the fetal up-take of glucose rely on maternal supply andtransplacental transport. Therefore, a defective placental transportersystem may affect the intrauterine environment compromising fetal aswell as mother well-being. On this line, we speculated that the placentalglucose transport system could be impaired in pre-eclampsia (PE).METHODS: Placentae were obtained after elective caesarean sectionsfollowing normal pregnancies and pre-eclamptic pregnancies. Syncytialbasal membrane (BM) and apical microvillus membrane (MVM) fractionswere prepared using differential ultra-centrifugation and magnesiumprecipitation. Protein expression was assessed by western blot. mRNAlevels were quantified by quantitative real-time PCR. A radiolabeledsubstrate up-take assay was established to assess glucose transport activity.FACS analysis was performed to check the shape of MVM. Statisticalanalysis was performed using one way ANOVA test.RESULTS: GLUT1 protein levels were down-regulated (70%; P<0.01)in pre-eclamptic placentae when compared to control placentae. This datais in line with the reduced glucose up-take in MVM prepared from preeclampticplacentae. Of note, the mRNA levels of GLUT1 did not changebetween placentae affected by PE and normal placentae, suggesting thatthe levels of GLUT1 are post-transcriptionally regulated. FACS analysison MVM vesicles from both normal placentae and pre-eclamptic placentaeshowed equal heterogeneity in the complexes formed. This excluded thepossibility that the altered glucose up-take observed in pre-eclampticMVM was caused by a different shape of these vesicles.CONCLUSIONS: Protein and functional studies of GLUT1 in MVMsuggest that in pre-eclampsia the glucose transport between mother andfetus might be defective. To further investigate this important biologicalaspect we will increase the number of samples obtained from patientsand use primary cells to study trans epithelial transport system in vitro." @default.
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- W2040641033 date "2013-09-01" @default.
- W2040641033 modified "2023-09-27" @default.
- W2040641033 title "Placental Glucose Transporter GLUT1 Expression in Pre-eclampsia" @default.
- W2040641033 doi "https://doi.org/10.1016/j.placenta.2013.06.262" @default.
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