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- W2040653013 abstract "We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future low-cost volume manufacturing. We report the separation of a nucleated cell fraction from cell debris and the bulk of the erythrocyte population, with the relatively rare (<2% starting concentration) NG2-positive cell population (pericytes and/or putative progenitor cells) being enriched up to 14-fold. This work demonstrates a potential clinical application for DEP-FFF and further establishes the utility of the method for achieving label-free fractionation of cell subpopulations." @default.
- W2040653013 created "2016-06-24" @default.
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- W2040653013 date "2008-01-01" @default.
- W2040653013 modified "2023-10-02" @default.
- W2040653013 title "Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation" @default.
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- W2040653013 doi "https://doi.org/10.1039/b717043b" @default.
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