Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040653123> ?p ?o ?g. }
- W2040653123 endingPage "38" @default.
- W2040653123 startingPage "31" @default.
- W2040653123 abstract "Many recombinant proteins carry an oligohistidine (His(X))-tag that allows their purification by immobilized metal affinity chromatography (IMAC). This tag can be exploited for the site-specific attachment of chromophores and fluorophores, using the same metal ion-nitrilotriacetic acid (NTA) coordination chemistry that forms the basis of popular versions of IMAC. Labeling proteins in this way can allow their detection at wavelengths outside of the absorption envelopes of un-modified proteins and nucleic acids. Here we describe use of this technology in tracer sedimentation experiments that can be performed in a standard analytical ultracentrifuge equipped with absorbance or fluorescence optics. Examples include sedimentation velocity in the presence of low molecular weight chromophoric solutes, sedimentation equilibrium in the presence of high concentrations of background protein and selective labeling to simplify the assignment of species in a complex interacting mixture." @default.
- W2040653123 created "2016-06-24" @default.
- W2040653123 creator A5007320411 @default.
- W2040653123 creator A5008707616 @default.
- W2040653123 creator A5021955814 @default.
- W2040653123 creator A5037484052 @default.
- W2040653123 creator A5039193076 @default.
- W2040653123 creator A5055618724 @default.
- W2040653123 creator A5085893830 @default.
- W2040653123 date "2011-05-01" @default.
- W2040653123 modified "2023-10-18" @default.
- W2040653123 title "Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge" @default.
- W2040653123 cites W1514670886 @default.
- W2040653123 cites W1565197201 @default.
- W2040653123 cites W1576360313 @default.
- W2040653123 cites W1596214091 @default.
- W2040653123 cites W17468178 @default.
- W2040653123 cites W1805506162 @default.
- W2040653123 cites W1967154908 @default.
- W2040653123 cites W1968623409 @default.
- W2040653123 cites W1969504883 @default.
- W2040653123 cites W1975317507 @default.
- W2040653123 cites W1976837580 @default.
- W2040653123 cites W1982626641 @default.
- W2040653123 cites W1984947863 @default.
- W2040653123 cites W1985007959 @default.
- W2040653123 cites W1985691286 @default.
- W2040653123 cites W1988141427 @default.
- W2040653123 cites W1988406719 @default.
- W2040653123 cites W1995275534 @default.
- W2040653123 cites W2002026561 @default.
- W2040653123 cites W200778230 @default.
- W2040653123 cites W2008466187 @default.
- W2040653123 cites W2016354183 @default.
- W2040653123 cites W2018252951 @default.
- W2040653123 cites W2023035897 @default.
- W2040653123 cites W2028622989 @default.
- W2040653123 cites W2033303857 @default.
- W2040653123 cites W2033676889 @default.
- W2040653123 cites W2034149051 @default.
- W2040653123 cites W2034968524 @default.
- W2040653123 cites W2039513725 @default.
- W2040653123 cites W2054629172 @default.
- W2040653123 cites W2057141116 @default.
- W2040653123 cites W2075145513 @default.
- W2040653123 cites W2076100371 @default.
- W2040653123 cites W2083576886 @default.
- W2040653123 cites W2094184203 @default.
- W2040653123 cites W2096814450 @default.
- W2040653123 cites W2098327367 @default.
- W2040653123 cites W2098744542 @default.
- W2040653123 cites W2100195390 @default.
- W2040653123 cites W2101002332 @default.
- W2040653123 cites W2105152313 @default.
- W2040653123 cites W2107295555 @default.
- W2040653123 cites W2107553595 @default.
- W2040653123 cites W2108305553 @default.
- W2040653123 cites W2108931507 @default.
- W2040653123 cites W2114522205 @default.
- W2040653123 cites W2122910625 @default.
- W2040653123 cites W2131187223 @default.
- W2040653123 cites W2135520740 @default.
- W2040653123 cites W2136498763 @default.
- W2040653123 cites W2152043216 @default.
- W2040653123 cites W2152322252 @default.
- W2040653123 cites W2163802608 @default.
- W2040653123 cites W2165360511 @default.
- W2040653123 cites W2165633081 @default.
- W2040653123 cites W2167241965 @default.
- W2040653123 cites W2172143141 @default.
- W2040653123 cites W4299496276 @default.
- W2040653123 doi "https://doi.org/10.1016/j.ymeth.2010.12.033" @default.
- W2040653123 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3090473" @default.
- W2040653123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21187151" @default.
- W2040653123 hasPublicationYear "2011" @default.
- W2040653123 type Work @default.
- W2040653123 sameAs 2040653123 @default.
- W2040653123 citedByCount "10" @default.
- W2040653123 countsByYear W20406531232012 @default.
- W2040653123 countsByYear W20406531232013 @default.
- W2040653123 countsByYear W20406531232014 @default.
- W2040653123 countsByYear W20406531232015 @default.
- W2040653123 countsByYear W20406531232016 @default.
- W2040653123 countsByYear W20406531232019 @default.
- W2040653123 crossrefType "journal-article" @default.
- W2040653123 hasAuthorship W2040653123A5007320411 @default.
- W2040653123 hasAuthorship W2040653123A5008707616 @default.
- W2040653123 hasAuthorship W2040653123A5021955814 @default.
- W2040653123 hasAuthorship W2040653123A5037484052 @default.
- W2040653123 hasAuthorship W2040653123A5039193076 @default.
- W2040653123 hasAuthorship W2040653123A5055618724 @default.
- W2040653123 hasAuthorship W2040653123A5085893830 @default.
- W2040653123 hasBestOaLocation W20406531232 @default.
- W2040653123 hasConcept C121332964 @default.
- W2040653123 hasConcept C178790620 @default.
- W2040653123 hasConcept C185592680 @default.
- W2040653123 hasConcept C192468462 @default.
- W2040653123 hasConcept C197404232 @default.