FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040653755> ?p ?o ?g. }

• W2040653755 endingPage "1252" @default.
• W2040653755 startingPage "1241" @default.
• W2040653755 abstract "The two-component system (TCS) composed of a pair of a sensor histidine kinase and a response regulator, allows bacteria to sense signals and respond to changes in their environment through specific gene activation or repression. The present study examined TCS in the obligatory intracellular bacteria Ehrlichia chaffeensis and Anaplasma phagocytophilum, that cause human monocytic ehrlichiosis (HME) and human granulocytic anaplasmosis (HGA) respectively. The genomes of E. chaffeensis and A. phagocytophilum were each predicted to encode three pairs of TCSs. All six genes encoding three histidine kinases and three response regulators were expressed in both E. chaffeensis and A. phagocytophilum cultured in human leukocytes. Pretreatment of host cell-free E. chaffeensis or A. phagocytophilum with closantel, an inhibitor of histidine kinases, completely blocked the infection of host cells. Treatment of infected cells 1 day post infection with closantel cleared infection in dose-dependent manner. All six genes in E. chaffeensis were cloned, recombinant proteins were expressed, and polyclonal antibodies were produced. Double immunofluorescence labelling and Western blot analysis revealed that all six proteins were expressed in cell culture. Autokinase activities of the three recombinant histidine kinases from E. chaffeensis were inhibited by closantel in vitro. A number of E. chaffeensis genes, including the six TCS genes, were downregulated within 5–60 min post closantel treatment. These results suggest that these TCSs play an essential role in infection and survival of E. chaffeensis and A. phagocytophilum in human leukocytes." @default.
• W2040653755 created "2016-06-24" @default.
• W2040653755 creator A5003482762 @default.
• W2040653755 creator A5016637080 @default.
• W2040653755 creator A5039483901 @default.
• W2040653755 creator A5048032078 @default.
• W2040653755 creator A5075487909 @default.
• W2040653755 date "2006-08-01" @default.
• W2040653755 modified "2023-10-15" @default.
• W2040653755 title "Intra-leukocyte expression of two-component systems in Ehrlichia chaffeensis and Anaplasma phagocytophilum and effects of the histidine kinase inhibitor closantel" @default.
• W2040653755 cites W1507853369 @default.
• W2040653755 cites W1547858367 @default.
• W2040653755 cites W1569904835 @default.
• W2040653755 cites W1654151873 @default.
• W2040653755 cites W1700547919 @default.
• W2040653755 cites W1888133447 @default.
• W2040653755 cites W1990657825 @default.
• W2040653755 cites W1999224877 @default.
• W2040653755 cites W2004938907 @default.
• W2040653755 cites W2006002169 @default.
• W2040653755 cites W2008122032 @default.
• W2040653755 cites W2027558100 @default.
• W2040653755 cites W2029889310 @default.
• W2040653755 cites W2032772890 @default.
• W2040653755 cites W2033169802 @default.
• W2040653755 cites W2033896866 @default.
• W2040653755 cites W2034266027 @default.
• W2040653755 cites W2043703947 @default.
• W2040653755 cites W2058681460 @default.
• W2040653755 cites W2061880687 @default.
• W2040653755 cites W2081945172 @default.
• W2040653755 cites W2088407530 @default.
• W2040653755 cites W2090635804 @default.
• W2040653755 cites W2106923147 @default.
• W2040653755 cites W2111866266 @default.
• W2040653755 cites W2116759905 @default.
• W2040653755 cites W2118291047 @default.
• W2040653755 cites W2122064356 @default.
• W2040653755 cites W2123799144 @default.
• W2040653755 cites W2123885130 @default.
• W2040653755 cites W2126161931 @default.
• W2040653755 cites W2131184698 @default.
• W2040653755 cites W2136856188 @default.
• W2040653755 cites W2139262051 @default.
• W2040653755 cites W2150580607 @default.
• W2040653755 cites W2155762470 @default.
• W2040653755 cites W2158016809 @default.
• W2040653755 cites W2160170449 @default.
• W2040653755 cites W2160765784 @default.
• W2040653755 cites W2161465177 @default.
• W2040653755 cites W2163117245 @default.
• W2040653755 cites W2163582500 @default.
• W2040653755 cites W2164101095 @default.
• W2040653755 cites W2170192876 @default.
• W2040653755 cites W4235589004 @default.
• W2040653755 doi "https://doi.org/10.1111/j.1462-5822.2006.00704.x" @default.
• W2040653755 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16882029" @default.
• W2040653755 hasPublicationYear "2006" @default.
• W2040653755 type Work @default.
• W2040653755 sameAs 2040653755 @default.
• W2040653755 citedByCount "58" @default.
• W2040653755 countsByYear W20406537552012 @default.
• W2040653755 countsByYear W20406537552013 @default.
• W2040653755 countsByYear W20406537552014 @default.
• W2040653755 countsByYear W20406537552015 @default.
• W2040653755 countsByYear W20406537552016 @default.
• W2040653755 countsByYear W20406537552017 @default.
• W2040653755 countsByYear W20406537552018 @default.
• W2040653755 countsByYear W20406537552019 @default.
• W2040653755 countsByYear W20406537552020 @default.
• W2040653755 countsByYear W20406537552021 @default.
• W2040653755 countsByYear W20406537552022 @default.
• W2040653755 countsByYear W20406537552023 @default.
• W2040653755 crossrefType "journal-article" @default.
• W2040653755 hasAuthorship W2040653755A5003482762 @default.
• W2040653755 hasAuthorship W2040653755A5016637080 @default.
• W2040653755 hasAuthorship W2040653755A5039483901 @default.
• W2040653755 hasAuthorship W2040653755A5048032078 @default.
• W2040653755 hasAuthorship W2040653755A5075487909 @default.
• W2040653755 hasBestOaLocation W20406537551 @default.
• W2040653755 hasConcept C104317684 @default.
• W2040653755 hasConcept C138223613 @default.
• W2040653755 hasConcept C143065580 @default.
• W2040653755 hasConcept C159047783 @default.
• W2040653755 hasConcept C159654299 @default.
• W2040653755 hasConcept C184235292 @default.
• W2040653755 hasConcept C2776370125 @default.
• W2040653755 hasConcept C2778905257 @default.
• W2040653755 hasConcept C2779159148 @default.
• W2040653755 hasConcept C2779620486 @default.
• W2040653755 hasConcept C2779657035 @default.
• W2040653755 hasConcept C2909063110 @default.
• W2040653755 hasConcept C54355233 @default.
• W2040653755 hasConcept C86803240 @default.
• W2040653755 hasConcept C89423630 @default.
• W2040653755 hasConceptScore W2040653755C104317684 @default.
• W2040653755 hasConceptScore W2040653755C138223613 @default.
• W2040653755 hasConceptScore W2040653755C143065580 @default.

• next