Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040656223> ?p ?o ?g. }
- W2040656223 endingPage "1877" @default.
- W2040656223 startingPage "1869" @default.
- W2040656223 abstract "Extract prepared from Xenopus eggs represents a cell-free system that has been shown to recapitulate a multitude of cellular processes, including cell cycle regulation, DNA replication/repair, and cytoskeletal dynamics. In addition, this system has been used to successfully reconstitute the Wnt pathway. Xenopus egg extract, which can be biochemically manipulated, offers an ideal medium in which small molecule screening can be performed in near native milieu. Thus, the use of Xenopus egg extract for small molecule screening represents an ideal bridge between targeted and phenotypic screening approaches. This review focuses on the use of this system for small molecules modulators of major signal transduction pathways (Notch, Hedgehog, and Wnt) that are critical for the development of the early Xenopus embryo. We describe the properties of Xenopus egg extract and our own high throughput screen for small molecules that modulate the Wnt pathway using this cell-free system. We propose that Xenopus egg extract could similarly be adapted for screening for modulators of the Notch and Hedgehog pathways." @default.
- W2040656223 created "2016-06-24" @default.
- W2040656223 creator A5023524466 @default.
- W2040656223 creator A5029225670 @default.
- W2040656223 creator A5041069157 @default.
- W2040656223 creator A5066896996 @default.
- W2040656223 creator A5081568154 @default.
- W2040656223 creator A5085015733 @default.
- W2040656223 date "2012-03-01" @default.
- W2040656223 modified "2023-10-16" @default.
- W2040656223 title "Screening for small molecule inhibitors of embryonic pathways: Sometimes you gotta crack a few eggs" @default.
- W2040656223 cites W116768201 @default.
- W2040656223 cites W1496078104 @default.
- W2040656223 cites W149621205 @default.
- W2040656223 cites W1552346480 @default.
- W2040656223 cites W1676048097 @default.
- W2040656223 cites W1861822938 @default.
- W2040656223 cites W1912203081 @default.
- W2040656223 cites W1963493384 @default.
- W2040656223 cites W1964793476 @default.
- W2040656223 cites W1965404941 @default.
- W2040656223 cites W1968682237 @default.
- W2040656223 cites W1972467203 @default.
- W2040656223 cites W1973569665 @default.
- W2040656223 cites W1974394081 @default.
- W2040656223 cites W1975492746 @default.
- W2040656223 cites W1975584335 @default.
- W2040656223 cites W1975993620 @default.
- W2040656223 cites W1976501146 @default.
- W2040656223 cites W1978583145 @default.
- W2040656223 cites W1980651213 @default.
- W2040656223 cites W1984379066 @default.
- W2040656223 cites W1985701763 @default.
- W2040656223 cites W1988501212 @default.
- W2040656223 cites W1988573682 @default.
- W2040656223 cites W1991101546 @default.
- W2040656223 cites W1994803892 @default.
- W2040656223 cites W1996984755 @default.
- W2040656223 cites W1997801717 @default.
- W2040656223 cites W2000589976 @default.
- W2040656223 cites W2001165707 @default.
- W2040656223 cites W2005717642 @default.
- W2040656223 cites W2006570444 @default.
- W2040656223 cites W2014871497 @default.
- W2040656223 cites W2015150670 @default.
- W2040656223 cites W2015436769 @default.
- W2040656223 cites W2019352743 @default.
- W2040656223 cites W2021124770 @default.
- W2040656223 cites W2021509469 @default.
- W2040656223 cites W2024236819 @default.
- W2040656223 cites W2025140711 @default.
- W2040656223 cites W2026379260 @default.
- W2040656223 cites W2029178312 @default.
- W2040656223 cites W2030923676 @default.
- W2040656223 cites W2033801528 @default.
- W2040656223 cites W2034373020 @default.
- W2040656223 cites W2037128138 @default.
- W2040656223 cites W2037450375 @default.
- W2040656223 cites W2044341552 @default.
- W2040656223 cites W2044616474 @default.
- W2040656223 cites W2044770435 @default.
- W2040656223 cites W2046104709 @default.
- W2040656223 cites W2046465935 @default.
- W2040656223 cites W2048990794 @default.
- W2040656223 cites W2050814521 @default.
- W2040656223 cites W2052214665 @default.
- W2040656223 cites W2054657634 @default.
- W2040656223 cites W2055330891 @default.
- W2040656223 cites W2055723335 @default.
- W2040656223 cites W2057830803 @default.
- W2040656223 cites W2059813946 @default.
- W2040656223 cites W2068072725 @default.
- W2040656223 cites W2069704514 @default.
- W2040656223 cites W2070127489 @default.
- W2040656223 cites W2071800444 @default.
- W2040656223 cites W2077040170 @default.
- W2040656223 cites W2077115549 @default.
- W2040656223 cites W2083425643 @default.
- W2040656223 cites W2086487946 @default.
- W2040656223 cites W2087131155 @default.
- W2040656223 cites W2093531978 @default.
- W2040656223 cites W2095217788 @default.
- W2040656223 cites W2099240520 @default.
- W2040656223 cites W2101859789 @default.
- W2040656223 cites W2103431676 @default.
- W2040656223 cites W2104510614 @default.
- W2040656223 cites W2105238625 @default.
- W2040656223 cites W2107332774 @default.
- W2040656223 cites W2107648723 @default.
- W2040656223 cites W2110708647 @default.
- W2040656223 cites W2112284540 @default.
- W2040656223 cites W2119284420 @default.
- W2040656223 cites W2119691744 @default.
- W2040656223 cites W2119780609 @default.
- W2040656223 cites W2126090986 @default.
- W2040656223 cites W2127001212 @default.
- W2040656223 cites W2133492742 @default.
- W2040656223 cites W2135763107 @default.