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• W2040657148 abstract "Herein, we report an anomalous electrochemical behavior of surface-bound DNA duplex that has single-base mismatches at its distal end. Single-stranded 15-base DNA was immobilized at its 5′end onto gold electrode surfaces. After hybridization with complementary or mismatched DNA, electrochemical impedance spectra were obtained using [Fe(CN)6]3−/4− as redox marker ions. Hybridization with the complementary DNA reduced the charge-transfer resistance (RCT), whereas single-base mismatches at the distal end of the duplex largely increased the RCT. This anomaly was found only with the distal end: the increase in RCT was not observed for mismatches at either the middle or the proximal end. These results indicate that electrochemical detection of single-base alterations at an end of sample DNA is exceptionally easy because of the diametrically opposite responses. This detection principle is promising for the typing of single-nucleotide polymorphisms in combination with the single-base primer extension protocol." @default.
• W2040657148 created "2016-06-24" @default.
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• W2040657148 date "2007-03-15" @default.
• W2040657148 modified "2023-09-25" @default.
• W2040657148 title "Detection of single-base mismatch at distal end of DNA duplex by electrochemical impedance spectroscopy" @default.
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• W2040657148 doi "https://doi.org/10.1016/j.bios.2006.08.008" @default.
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