FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040672289> ?p ?o ?g. }

• W2040672289 abstract "The liver is a primary site of metastasis for some of the most common human malignancies. At present, surgical resection is the most effective curative option for liver metastases, but most patients with liver metastases still succumb to their disease. A better understanding of the underlying biology is essential for design of more effective therapy. We previously identified basement membrane type IV collagen α1/α2 expression levels as determinants of the liver colonizing potential in a murine lung carcinoma model (1) and observed high collagen IV expression levels in surgical specimens of liver metastases from diverse tumor types. The aim of this study was to elucidate the functional relevance of increased collagen IV expression to liver metastases formation. We reported that collagen IV α1/α2 overexpression in non-metastatic lung carcinoma M-27 (M27colIV) cells increased specifically their liver (but not lung) metastasizing potential. A transcriptome analysis was therefore performed on these cells in order to identify changes to gene expression that could account for the newly acquired metastatic potential. This analysis revealed that type IV collagen levels regulated the expression of multiple genes. Prominent among them were genes encoding for chemokines such as CCL-5 and CCL-7 and for growth factors such as amphiregulin (AREG) that were all upregulated by ≥ 3 fold. These changes were validated at the RNA and protein levels using qPCR and Western blotting, respectively. When the expression of these genes in M27colIV cells was subsequently silenced using shRNA, a significant decrease (5-10 folds) was observed in their ability to generate experimental liver metastases, as compared to wild type or mock-transfected cells and they failed to develop metastases in 40-50 % of injected mice. A similar correlation between CCL-5 and CCL-7 expression levels and liver metastases formation was also noted in human colon carcinoma cell lines. The results identify a type IV collagen-regulated gene expression signature that promotes liver metastasis and suggest that collagen IV-induced changes in chemokine and growth factor production levels mediate this effect. They provide a rationale for further exploration of the clinical utility of CCL-5, CCL-7 and AREG as targets in the management of liver metastases. Supported by a grant from the Canadian Institute for Health Research (to PB) and a Henry R. Shibata Cedars Cancer Fellowship (to RR). REFERENCES 1. Burnier JV, Wang N, Michel RP, Hassanain M, Li S, Lu Y, et al. Type IV collagen-initiated signals provide survival and growth cues required for liver metastasis. Oncogene. 2011;30:3766-83. Citation Format: Roni F. Rayes, Ni Wang, Julia V. Burnier, France Bourdeau, Pnina Brodt. Regulation of site-specific liver metastasis by collagen IV-conveyed signals. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2094. doi:10.1158/1538-7445.AM2014-2094" @default.
• W2040672289 created "2016-06-24" @default.
• W2040672289 creator A5019820429 @default.
• W2040672289 creator A5049237612 @default.
• W2040672289 creator A5056416945 @default.
• W2040672289 creator A5086581000 @default.
• W2040672289 date "2014-09-30" @default.
• W2040672289 modified "2023-09-27" @default.
• W2040672289 title "Abstract 2094: Regulation of site-specific liver metastasis by collagen IV-conveyed signals" @default.
• W2040672289 doi "https://doi.org/10.1158/1538-7445.am2014-2094" @default.
• W2040672289 hasPublicationYear "2014" @default.
• W2040672289 type Work @default.
• W2040672289 sameAs 2040672289 @default.
• W2040672289 citedByCount "0" @default.
• W2040672289 crossrefType "proceedings-article" @default.
• W2040672289 hasAuthorship W2040672289A5019820429 @default.
• W2040672289 hasAuthorship W2040672289A5049237612 @default.
• W2040672289 hasAuthorship W2040672289A5056416945 @default.
• W2040672289 hasAuthorship W2040672289A5086581000 @default.
• W2040672289 hasConcept C104317684 @default.
• W2040672289 hasConcept C121608353 @default.
• W2040672289 hasConcept C126322002 @default.
• W2040672289 hasConcept C142724271 @default.
• W2040672289 hasConcept C150194340 @default.
• W2040672289 hasConcept C162317418 @default.
• W2040672289 hasConcept C189165786 @default.
• W2040672289 hasConcept C190232843 @default.
• W2040672289 hasConcept C2777107211 @default.
• W2040672289 hasConcept C2777546739 @default.
• W2040672289 hasConcept C2777714996 @default.
• W2040672289 hasConcept C2779013556 @default.
• W2040672289 hasConcept C2779438470 @default.
• W2040672289 hasConcept C2779814568 @default.
• W2040672289 hasConcept C2780806702 @default.
• W2040672289 hasConcept C502942594 @default.
• W2040672289 hasConcept C55493867 @default.
• W2040672289 hasConcept C67705224 @default.
• W2040672289 hasConcept C71924100 @default.
• W2040672289 hasConcept C85265743 @default.
• W2040672289 hasConcept C86803240 @default.
• W2040672289 hasConcept C95444343 @default.
• W2040672289 hasConceptScore W2040672289C104317684 @default.
• W2040672289 hasConceptScore W2040672289C121608353 @default.
• W2040672289 hasConceptScore W2040672289C126322002 @default.
• W2040672289 hasConceptScore W2040672289C142724271 @default.
• W2040672289 hasConceptScore W2040672289C150194340 @default.
• W2040672289 hasConceptScore W2040672289C162317418 @default.
• W2040672289 hasConceptScore W2040672289C189165786 @default.
• W2040672289 hasConceptScore W2040672289C190232843 @default.
• W2040672289 hasConceptScore W2040672289C2777107211 @default.
• W2040672289 hasConceptScore W2040672289C2777546739 @default.
• W2040672289 hasConceptScore W2040672289C2777714996 @default.
• W2040672289 hasConceptScore W2040672289C2779013556 @default.
• W2040672289 hasConceptScore W2040672289C2779438470 @default.
• W2040672289 hasConceptScore W2040672289C2779814568 @default.
• W2040672289 hasConceptScore W2040672289C2780806702 @default.
• W2040672289 hasConceptScore W2040672289C502942594 @default.
• W2040672289 hasConceptScore W2040672289C55493867 @default.
• W2040672289 hasConceptScore W2040672289C67705224 @default.
• W2040672289 hasConceptScore W2040672289C71924100 @default.
• W2040672289 hasConceptScore W2040672289C85265743 @default.
• W2040672289 hasConceptScore W2040672289C86803240 @default.
• W2040672289 hasConceptScore W2040672289C95444343 @default.
• W2040672289 hasLocation W20406722891 @default.
• W2040672289 hasOpenAccess W2040672289 @default.
• W2040672289 hasPrimaryLocation W20406722891 @default.
• W2040672289 hasRelatedWork W1594294131 @default.
• W2040672289 hasRelatedWork W1999306772 @default.
• W2040672289 hasRelatedWork W2002105749 @default.
• W2040672289 hasRelatedWork W2005715557 @default.
• W2040672289 hasRelatedWork W2042080657 @default.
• W2040672289 hasRelatedWork W2080203781 @default.
• W2040672289 hasRelatedWork W2090859076 @default.
• W2040672289 hasRelatedWork W2158683414 @default.
• W2040672289 hasRelatedWork W2294548748 @default.
• W2040672289 hasRelatedWork W2320081409 @default.
• W2040672289 hasRelatedWork W2739935096 @default.
• W2040672289 hasRelatedWork W2793350609 @default.
• W2040672289 hasRelatedWork W2944802085 @default.
• W2040672289 hasRelatedWork W2954037475 @default.
• W2040672289 hasRelatedWork W2954566482 @default.
• W2040672289 hasRelatedWork W2956131920 @default.
• W2040672289 hasRelatedWork W2979969859 @default.
• W2040672289 hasRelatedWork W3048789412 @default.
• W2040672289 hasRelatedWork W3205433204 @default.
• W2040672289 hasRelatedWork W3211858175 @default.
• W2040672289 isParatext "false" @default.
• W2040672289 isRetracted "false" @default.
• W2040672289 magId "2040672289" @default.
• W2040672289 workType "article" @default.