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• W2040679936 abstract "1 Sonication, freezing and thawing, treatment of mitochondria with Tween-Span surfactants and other procedures applied to isolate ferrochelatase from the liver mitochondria showed that the most active in releasing the enzyme was the combined action of Tween 20 at a final concentration of 15 mg/ml and sonic irradiation on the mitochondrial suspensions, previously subjected to freezing and thawing. 2 A 20–40 fold purification of the enzyme was achieved by ammonium sulphate fractionation and chromatography on dextran gels of “solubilized” ferrochelatase. 3 Variations of the yield of the release of ferrochelatase from the mitochondria suggest that the localization of the enzyme within the mitochondrial structure is heterogeneous." @default.
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• W2040679936 date "2005-03-03" @default.
• W2040679936 modified "2023-09-26" @default.
• W2040679936 title "Chelation of Iron and Zinc by Protoporphyrin Catalyzed by Mitochondrial Preparations" @default.
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• W2040679936 doi "https://doi.org/10.1111/j.1432-1033.1969.tb19646.x" @default.
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