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- W2040685993 abstract "Abstract The post-illumination photohemolysis of human red blood cells was used as a model system for studying membrane damage sensitized by various phthalocyanine sulfonates. With a constant fluence and photolysis time at 670 ± 10 nm and with a fixed optical density of the sensitizer, the percentage of hemolysis was measured as a function of time after illumination; and from the usual sigmoidal shaped curves, the times for 50% hemolysis were determined. The most effective central cation was Al, followed by Zn; the metal-free compound and those containing the paramagnetic ions Cu, Fe and Co were inactive. The sensitizer bound to the red blood cells was found to be effective for photohemolysis. Studies of the Al compound in 98.0% deuterated media (as measured by NMR) showed a definite deuterium isotope effect, suggesting the participation of singlet oxygen. This result was supported by the large protective effects of 3.3 raM and 13.3 mM tryptophan present during photolysis. Tryptophan reacts rapidly with singlet oxygen and with OH radicals. The much smaller effects observed with the same concentrations of mannitol and glycerol, OH radical scavengers which do not react with singlet oxygen, are consistent with the predominant role of singlet oxygen in photohemolysis sensitized by Al phthalocyanine sulfonate." @default.
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- W2040685993 date "1987-11-01" @default.
- W2040685993 modified "2023-09-27" @default.
- W2040685993 title "THE ROLE OF SINGLET OXYGEN IN THE PHOTOHEMOLYSIS OF RED BLOOD CELLS SENSITIZED BY PHTHALOCYANINE SULFONATES" @default.
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- W2040685993 doi "https://doi.org/10.1111/j.1751-1097.1987.tb04823.x" @default.
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