FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040700314> ?p ?o ?g. }

• W2040700314 endingPage "425" @default.
• W2040700314 startingPage "414" @default.
• W2040700314 abstract "Formation of the 4-kDa peptides, which are essential constituents of the extracellular plaques in Alzheimer's disease, involves the sequential cleavage of the amyloid precursor protein (APP) by beta- and gamma-secretases. The carboxy-terminal 99-amino-acid peptide which is liberated from APP by beta-secretase was used as a potential native substrate of the gamma-secretase(s). With the addition of an initiator Met and a FLAG sequence at the C-terminus (betaAPP100-FLAG), it was expressed in Escherichia coli under the control of the T7 promotor. The preferred site(s) of cleavage in the N-terminal 40-amino-acid beta-amyloid peptide and betaAPP100-FLAG by potential gamma-secretase(s) were rapidly identified using matrix-assisted laser-desorption/ionization time-of-flight mass spectroscopy in addition to peptide mapping followed by protein sequence analysis. Since gamma-secretases seem to be active at acidic pH, three cathepsins (D, E and B) were selected for testing. Studies using different detergents indicated that the cleavage preference of cathepsin D for the betaAPP100-FLAG is highly dependent on the surfactant used to solubilize this substrate. All three cathepsins were found to be capable of catabolizing both beta-amyloid peptides and the betaAPP100-FLAG. As cathepsin D was found to cleave the betaAPP100-FLAG in the vicinity of the C-terminus of the beta-amyloid peptides and cathepsin B has a high carboxypeptidase activity at low pH, the possibility cannot be excluded that cathepsins D and B are involved in the amyloidogenic processing of APP." @default.
• W2040700314 created "2016-06-24" @default.
• W2040700314 creator A5006200348 @default.
• W2040700314 creator A5006700105 @default.
• W2040700314 creator A5007452889 @default.
• W2040700314 creator A5009733209 @default.
• W2040700314 creator A5010920048 @default.
• W2040700314 creator A5021389035 @default.
• W2040700314 creator A5024711246 @default.
• W2040700314 creator A5028814613 @default.
• W2040700314 creator A5034145050 @default.
• W2040700314 creator A5040989348 @default.
• W2040700314 creator A5056964019 @default.
• W2040700314 creator A5063514591 @default.
• W2040700314 creator A5071866756 @default.
• W2040700314 creator A5073773810 @default.
• W2040700314 creator A5078244753 @default.
• W2040700314 date "1997-03-01" @default.
• W2040700314 modified "2023-09-26" @default.
• W2040700314 title "A Possible Role for Cathepsins D, E, and B in the Processing of beta-amyloid Precursor Protein in Alzheimer's Disease" @default.
• W2040700314 cites W1124073478 @default.
• W2040700314 cites W1483408272 @default.
• W2040700314 cites W1484675846 @default.
• W2040700314 cites W1484740986 @default.
• W2040700314 cites W1485742470 @default.
• W2040700314 cites W1496807705 @default.
• W2040700314 cites W1504320357 @default.
• W2040700314 cites W1521304851 @default.
• W2040700314 cites W1525574052 @default.
• W2040700314 cites W1555196812 @default.
• W2040700314 cites W1562575254 @default.
• W2040700314 cites W1614175121 @default.
• W2040700314 cites W1773701686 @default.
• W2040700314 cites W1845792010 @default.
• W2040700314 cites W1877959507 @default.
• W2040700314 cites W1966936332 @default.
• W2040700314 cites W1969018508 @default.
• W2040700314 cites W1973573196 @default.
• W2040700314 cites W1974302575 @default.
• W2040700314 cites W1974928505 @default.
• W2040700314 cites W1975993749 @default.
• W2040700314 cites W1980749733 @default.
• W2040700314 cites W1988022242 @default.
• W2040700314 cites W1988731759 @default.
• W2040700314 cites W1990818761 @default.
• W2040700314 cites W1996574051 @default.
• W2040700314 cites W2009246830 @default.
• W2040700314 cites W2011619359 @default.
• W2040700314 cites W2015481531 @default.
• W2040700314 cites W2018145486 @default.
• W2040700314 cites W2020235691 @default.
• W2040700314 cites W2032920422 @default.
• W2040700314 cites W2038012168 @default.
• W2040700314 cites W2038086035 @default.
• W2040700314 cites W2039035040 @default.
• W2040700314 cites W2046847750 @default.
• W2040700314 cites W2048506492 @default.
• W2040700314 cites W2053413315 @default.
• W2040700314 cites W2064473937 @default.
• W2040700314 cites W2070810383 @default.
• W2040700314 cites W2071691905 @default.
• W2040700314 cites W2076305899 @default.
• W2040700314 cites W2077370394 @default.
• W2040700314 cites W2081678091 @default.
• W2040700314 cites W2081881251 @default.
• W2040700314 cites W2082504479 @default.
• W2040700314 cites W2084754412 @default.
• W2040700314 cites W2087255758 @default.
• W2040700314 cites W2090553671 @default.
• W2040700314 cites W2091550286 @default.
• W2040700314 cites W2100837269 @default.
• W2040700314 cites W2101880944 @default.
• W2040700314 cites W2130144581 @default.
• W2040700314 cites W2150455571 @default.
• W2040700314 cites W2153701967 @default.
• W2040700314 cites W2162633850 @default.
• W2040700314 cites W2165449806 @default.
• W2040700314 cites W2505247346 @default.
• W2040700314 doi "https://doi.org/10.1111/j.1432-1033.1997.00414.x" @default.
• W2040700314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9119007" @default.
• W2040700314 hasPublicationYear "1997" @default.
• W2040700314 type Work @default.
• W2040700314 sameAs 2040700314 @default.
• W2040700314 citedByCount "84" @default.
• W2040700314 countsByYear W20407003142012 @default.
• W2040700314 countsByYear W20407003142013 @default.
• W2040700314 countsByYear W20407003142014 @default.
• W2040700314 countsByYear W20407003142016 @default.
• W2040700314 countsByYear W20407003142017 @default.
• W2040700314 countsByYear W20407003142018 @default.
• W2040700314 countsByYear W20407003142019 @default.
• W2040700314 countsByYear W20407003142020 @default.
• W2040700314 countsByYear W20407003142021 @default.
• W2040700314 countsByYear W20407003142022 @default.
• W2040700314 countsByYear W20407003142023 @default.
• W2040700314 crossrefType "journal-article" @default.
• W2040700314 hasAuthorship W2040700314A5006200348 @default.
• W2040700314 hasAuthorship W2040700314A5006700105 @default.

• next