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• W2040708730 endingPage "e27719" @default.
• W2040708730 startingPage "e27719" @default.
• W2040708730 abstract "The in vivo studies of myocardial infarct using c-kit+/Lin− cardiac stem cells (CSCs) are still in the early stage with margin or no beneficial effects for cardiac function. One of the potential reasons may be related to the absence of fully understanding the properties of these cells both in vitro and in vivo. In the present study, we aimed to systematically examine how CSCs adapted to in vitro cell processes and whether there is any cell contamination after long-term culture. Human CSCs were enzymatically isolated from the atrial appendages of patients. The fixed tissue sections, freshly isolated or cultured CSCs were then used for identification of c-kit+/Lin− cells, detection of cell contamination, or differentiation of cardiac lineages. By specific antibody staining, we demonstrated that tissue sections from atrial appendages contained less than 0.036% c-kit+/Lin− cells. For the first time, we noted that without magnetic activated cell sorting (MACS), the percentages of c-kit+/Lin− cells gradually increased up to ∼40% during continuously culture between passage 2 to 8, but could not exceed >80% unless c-kit MACS was carried out. The resulting c-kit+/Lin− cells were negative for CD34, CD45, CD133, and Lin markers, but positive for KDR and CD31 in few patients after c-kit MACS. Lin depletion seemed unnecessary for enrichment of c-kit+/Lin− cell population. Following induced differentiation, c-kit+/Lin− CSCs demonstrated strong differentiation towards cardiomyocytes but less towards smooth and endothelial cells. We concluded that by using an enzymatic dissociation method, a large number, or higher percentage, of relative pure human CSCs with stable expression of c-kit+ could be obtained from atrial appendage specimens within ∼4 weeks following c-kit MACS without Lin depletion. This simple but cost-effective approach can be used to obtain enough numbers of stably-expressed c-kit+/Lin− cells for clinical trials in repairing myocardial infarction." @default.
• W2040708730 created "2016-06-24" @default.
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• W2040708730 date "2011-11-28" @default.
• W2040708730 modified "2023-09-28" @default.
• W2040708730 title "Human Cardiac Stem Cells Isolated from Atrial Appendages Stably Express c-kit" @default.
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• W2040708730 cites W1970661970 @default.
• W2040708730 cites W1982584865 @default.
• W2040708730 cites W1984514695 @default.
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• W2040708730 cites W2029601087 @default.
• W2040708730 cites W2032395940 @default.
• W2040708730 cites W2034998169 @default.
• W2040708730 cites W2037234506 @default.
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• W2040708730 cites W2075754372 @default.
• W2040708730 cites W2080539468 @default.
• W2040708730 cites W2081066133 @default.
• W2040708730 cites W2081559723 @default.
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• W2040708730 doi "https://doi.org/10.1371/journal.pone.0027719" @default.
• W2040708730 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3225366" @default.
• W2040708730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22140461" @default.
• W2040708730 hasPublicationYear "2011" @default.
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