Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040744098> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2040744098 endingPage "368" @default.
- W2040744098 startingPage "362" @default.
- W2040744098 abstract "Most commonly used surfactants were found to be inhibitors of partially purified rat brain protein kinase C at or above their critical micellar concentrations (CMC). These include sodium lauryl sulfate, deoxycholate, octyl glucoside, dodecyl trimethylammonium bromide, linear alkylbenzene sulfonate and Triton X-100. Several detergents, including the nonionic surfactants digitonin and Neodol-12 (ethoxylated alcohol), did not inhibit protein kinase C activity, even at concentrations greater than their CMC, while the anionic surfactant, AEOS-12 (ethoxylated alcohol sulfate), inhibited enzyme activity only slightly (less than 8%). Since these latter surfactants have little or no inhibitory effect on protein kinase C, they may be of value in solubilizing cells and tissues for the determination of enzyme activity in crude extracts. Among the detergents tested, sodium lauryl sulfate and linear alkylbenzene sulfonate significantly stimulated protein kinase C activity in the absence of phosphatidylserine and calcium. This was found to be dependent on the presence of histone in the protein kinase C assay. These detergents failed to stimulate protein kinase C activity when endogenous proteins in the partially purified rat brain extracts were used as the substrate. Our results indicate that activity of protein kinase C can be modified by the conditions of the assay and by the detergents used to extract the enzyme." @default.
- W2040744098 created "2016-06-24" @default.
- W2040744098 creator A5000330812 @default.
- W2040744098 creator A5012602325 @default.
- W2040744098 creator A5072192770 @default.
- W2040744098 creator A5081341006 @default.
- W2040744098 creator A5083821495 @default.
- W2040744098 date "1989-09-01" @default.
- W2040744098 modified "2023-09-27" @default.
- W2040744098 title "Ethoxylated alcohol (Neodol-12) and other surfactants in the assay of protein kinase C" @default.
- W2040744098 cites W1486602990 @default.
- W2040744098 cites W1588018069 @default.
- W2040744098 cites W1594758412 @default.
- W2040744098 cites W1595529083 @default.
- W2040744098 cites W1878167671 @default.
- W2040744098 cites W191339276 @default.
- W2040744098 cites W1973005880 @default.
- W2040744098 cites W1973179766 @default.
- W2040744098 cites W1974190630 @default.
- W2040744098 cites W1978060911 @default.
- W2040744098 cites W1989574638 @default.
- W2040744098 cites W1989675515 @default.
- W2040744098 cites W1997833187 @default.
- W2040744098 cites W2001692359 @default.
- W2040744098 cites W2006858866 @default.
- W2040744098 cites W2016222043 @default.
- W2040744098 cites W2036079185 @default.
- W2040744098 cites W2077623770 @default.
- W2040744098 cites W2100837269 @default.
- W2040744098 cites W2232067801 @default.
- W2040744098 cites W283232088 @default.
- W2040744098 cites W4293247451 @default.
- W2040744098 cites W63406335 @default.
- W2040744098 doi "https://doi.org/10.1016/0304-4165(89)90097-4" @default.
- W2040744098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2775791" @default.
- W2040744098 hasPublicationYear "1989" @default.
- W2040744098 type Work @default.
- W2040744098 sameAs 2040744098 @default.
- W2040744098 citedByCount "6" @default.
- W2040744098 crossrefType "journal-article" @default.
- W2040744098 hasAuthorship W2040744098A5000330812 @default.
- W2040744098 hasAuthorship W2040744098A5012602325 @default.
- W2040744098 hasAuthorship W2040744098A5072192770 @default.
- W2040744098 hasAuthorship W2040744098A5081341006 @default.
- W2040744098 hasAuthorship W2040744098A5083821495 @default.
- W2040744098 hasConcept C11268172 @default.
- W2040744098 hasConcept C178790620 @default.
- W2040744098 hasConcept C181199279 @default.
- W2040744098 hasConcept C184235292 @default.
- W2040744098 hasConcept C184651966 @default.
- W2040744098 hasConcept C185592680 @default.
- W2040744098 hasConcept C195794163 @default.
- W2040744098 hasConcept C2776300020 @default.
- W2040744098 hasConcept C2778913249 @default.
- W2040744098 hasConcept C2778920010 @default.
- W2040744098 hasConcept C43617362 @default.
- W2040744098 hasConcept C55493867 @default.
- W2040744098 hasConcept C58226133 @default.
- W2040744098 hasConcept C64237528 @default.
- W2040744098 hasConcept C97029542 @default.
- W2040744098 hasConceptScore W2040744098C11268172 @default.
- W2040744098 hasConceptScore W2040744098C178790620 @default.
- W2040744098 hasConceptScore W2040744098C181199279 @default.
- W2040744098 hasConceptScore W2040744098C184235292 @default.
- W2040744098 hasConceptScore W2040744098C184651966 @default.
- W2040744098 hasConceptScore W2040744098C185592680 @default.
- W2040744098 hasConceptScore W2040744098C195794163 @default.
- W2040744098 hasConceptScore W2040744098C2776300020 @default.
- W2040744098 hasConceptScore W2040744098C2778913249 @default.
- W2040744098 hasConceptScore W2040744098C2778920010 @default.
- W2040744098 hasConceptScore W2040744098C43617362 @default.
- W2040744098 hasConceptScore W2040744098C55493867 @default.
- W2040744098 hasConceptScore W2040744098C58226133 @default.
- W2040744098 hasConceptScore W2040744098C64237528 @default.
- W2040744098 hasConceptScore W2040744098C97029542 @default.
- W2040744098 hasIssue "3" @default.
- W2040744098 hasLocation W20407440981 @default.
- W2040744098 hasLocation W20407440982 @default.
- W2040744098 hasOpenAccess W2040744098 @default.
- W2040744098 hasPrimaryLocation W20407440981 @default.
- W2040744098 hasRelatedWork W1559861641 @default.
- W2040744098 hasRelatedWork W1981729706 @default.
- W2040744098 hasRelatedWork W2003689538 @default.
- W2040744098 hasRelatedWork W2010372362 @default.
- W2040744098 hasRelatedWork W2033240963 @default.
- W2040744098 hasRelatedWork W2047677460 @default.
- W2040744098 hasRelatedWork W2062454642 @default.
- W2040744098 hasRelatedWork W2094979353 @default.
- W2040744098 hasRelatedWork W2378042071 @default.
- W2040744098 hasRelatedWork W4289960061 @default.
- W2040744098 hasVolume "992" @default.
- W2040744098 isParatext "false" @default.
- W2040744098 isRetracted "false" @default.
- W2040744098 magId "2040744098" @default.
- W2040744098 workType "article" @default.