Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040802461> ?p ?o ?g. }
- W2040802461 endingPage "56" @default.
- W2040802461 startingPage "52" @default.
- W2040802461 abstract "The availability of fetal cells from the maternal peripheral blood opens up the possibility for a noninvasive prenatal diagnosis. Until now this prospect has been hampered by the fact that there are no highly specific antibodies to fetal cells. In the present study, four as yet untested monoclonal antibodies - 2G5, 3F9, EPO-R and Flk1 - were analyzed in view of their effectiveness to stain fetal nucleated cells. Therefore, 20 ml of peripheral blood from 40 women carrying a male fetus were collected in the 16th to 28th weeks of pregnancy. Mononucleated cells, enriched by density gradient, were stained with one of the four antibodies and isolated with magnetic cell sorting. Subsequently, fluorescence in situ hybridization with chromosome X and Y centromere probes was performed which allowed us to analyze the maternal or fetal origin of the enriched cells. For evaluation, 500 nuclei per case were examined. The antibody 3F9 detected 0.2 cells on average (n = 5), 2G5 1.2 (n = 11), EPO-R 1.6 (n = 10) and Flk1 1.8 cells (n = 8) with fetal specific Y-positive hybridization signals. The antibody against CD71 (n = 6) used as control gave no Y-specific signals. Considering these results, the tested antibodies, especially Flk1, appear to be more successful in detecting fetal cells than the commonly used CD71 antibody." @default.
- W2040802461 created "2016-06-24" @default.
- W2040802461 creator A5015346266 @default.
- W2040802461 creator A5037374768 @default.
- W2040802461 creator A5037645126 @default.
- W2040802461 creator A5061899849 @default.
- W2040802461 creator A5086005802 @default.
- W2040802461 creator A5086391995 @default.
- W2040802461 date "2000-12-15" @default.
- W2040802461 modified "2023-09-25" @default.
- W2040802461 title "Applicability of Four New Antibodies for the Detection of Fetal Nucleated Cells Out of Maternal Blood by FISH Analysis" @default.
- W2040802461 cites W1528652392 @default.
- W2040802461 cites W1852654097 @default.
- W2040802461 cites W1965281109 @default.
- W2040802461 cites W1970855306 @default.
- W2040802461 cites W1972794925 @default.
- W2040802461 cites W1986570408 @default.
- W2040802461 cites W1987525435 @default.
- W2040802461 cites W1988425746 @default.
- W2040802461 cites W1992965066 @default.
- W2040802461 cites W1994764480 @default.
- W2040802461 cites W2005571348 @default.
- W2040802461 cites W2007373720 @default.
- W2040802461 cites W2013050080 @default.
- W2040802461 cites W2014720310 @default.
- W2040802461 cites W2016484985 @default.
- W2040802461 cites W2032285829 @default.
- W2040802461 cites W2051755643 @default.
- W2040802461 cites W2058287322 @default.
- W2040802461 cites W2066796679 @default.
- W2040802461 cites W2069013751 @default.
- W2040802461 cites W2072721747 @default.
- W2040802461 cites W2074912037 @default.
- W2040802461 cites W2084949292 @default.
- W2040802461 cites W2104513825 @default.
- W2040802461 cites W2105179039 @default.
- W2040802461 cites W2120315162 @default.
- W2040802461 cites W2128564585 @default.
- W2040802461 cites W2145903120 @default.
- W2040802461 cites W2168736535 @default.
- W2040802461 cites W2415436678 @default.
- W2040802461 doi "https://doi.org/10.1159/000053881" @default.
- W2040802461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11125253" @default.
- W2040802461 hasPublicationYear "2000" @default.
- W2040802461 type Work @default.
- W2040802461 sameAs 2040802461 @default.
- W2040802461 citedByCount "4" @default.
- W2040802461 crossrefType "journal-article" @default.
- W2040802461 hasAuthorship W2040802461A5015346266 @default.
- W2040802461 hasAuthorship W2040802461A5037374768 @default.
- W2040802461 hasAuthorship W2040802461A5037645126 @default.
- W2040802461 hasAuthorship W2040802461A5061899849 @default.
- W2040802461 hasAuthorship W2040802461A5086005802 @default.
- W2040802461 hasAuthorship W2040802461A5086391995 @default.
- W2040802461 hasConcept C104317684 @default.
- W2040802461 hasConcept C142724271 @default.
- W2040802461 hasConcept C153911025 @default.
- W2040802461 hasConcept C159654299 @default.
- W2040802461 hasConcept C16685009 @default.
- W2040802461 hasConcept C172680121 @default.
- W2040802461 hasConcept C203014093 @default.
- W2040802461 hasConcept C2777542201 @default.
- W2040802461 hasConcept C2778258057 @default.
- W2040802461 hasConcept C2778630268 @default.
- W2040802461 hasConcept C2779234561 @default.
- W2040802461 hasConcept C2910929737 @default.
- W2040802461 hasConcept C30481170 @default.
- W2040802461 hasConcept C35866371 @default.
- W2040802461 hasConcept C542903549 @default.
- W2040802461 hasConcept C54355233 @default.
- W2040802461 hasConcept C553184892 @default.
- W2040802461 hasConcept C71924100 @default.
- W2040802461 hasConcept C86803240 @default.
- W2040802461 hasConceptScore W2040802461C104317684 @default.
- W2040802461 hasConceptScore W2040802461C142724271 @default.
- W2040802461 hasConceptScore W2040802461C153911025 @default.
- W2040802461 hasConceptScore W2040802461C159654299 @default.
- W2040802461 hasConceptScore W2040802461C16685009 @default.
- W2040802461 hasConceptScore W2040802461C172680121 @default.
- W2040802461 hasConceptScore W2040802461C203014093 @default.
- W2040802461 hasConceptScore W2040802461C2777542201 @default.
- W2040802461 hasConceptScore W2040802461C2778258057 @default.
- W2040802461 hasConceptScore W2040802461C2778630268 @default.
- W2040802461 hasConceptScore W2040802461C2779234561 @default.
- W2040802461 hasConceptScore W2040802461C2910929737 @default.
- W2040802461 hasConceptScore W2040802461C30481170 @default.
- W2040802461 hasConceptScore W2040802461C35866371 @default.
- W2040802461 hasConceptScore W2040802461C542903549 @default.
- W2040802461 hasConceptScore W2040802461C54355233 @default.
- W2040802461 hasConceptScore W2040802461C553184892 @default.
- W2040802461 hasConceptScore W2040802461C71924100 @default.
- W2040802461 hasConceptScore W2040802461C86803240 @default.
- W2040802461 hasIssue "1" @default.
- W2040802461 hasLocation W20408024611 @default.
- W2040802461 hasLocation W20408024612 @default.
- W2040802461 hasOpenAccess W2040802461 @default.
- W2040802461 hasPrimaryLocation W20408024611 @default.
- W2040802461 hasRelatedWork W127720800 @default.