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• W2040805829 abstract "Next Generation Sequencing (NGS) is producing enormous corpuses of short DNA reads, affecting emerging fields like metagenomics. Protein similarity search--a key step to achieve annotation of protein-coding genes in these short reads, and identification of their biological functions--faces daunting challenges because of the very sizes of the short read datasets. We developed a fast protein similarity search tool RAPSearch that utilizes a reduced amino acid alphabet and suffix array to detect seeds of flexible length. For short reads (translated in 6 frames) we tested, RAPSearch achieved ~20-90 times speedup as compared to BLASTX. RAPSearch missed only a small fraction (~1.3-3.2%) of BLASTX similarity hits, but it also discovered additional homologous proteins (~0.3-2.1%) that BLASTX missed. By contrast, BLAT, a tool that is even slightly faster than RAPSearch, had significant loss of sensitivity as compared to RAPSearch and BLAST. RAPSearch is implemented as open-source software and is accessible at http://omics.informatics.indiana.edu/mg/RAPSearch . It enables faster protein similarity search. The application of RAPSearch in metageomics has also been demonstrated." @default.
• W2040805829 created "2016-06-24" @default.
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• W2040805829 date "2011-05-15" @default.
• W2040805829 modified "2023-10-14" @default.
• W2040805829 title "RAPSearch: a fast protein similarity search tool for short reads" @default.
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• W2040805829 doi "https://doi.org/10.1186/1471-2105-12-159" @default.
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