FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040809736> ?p ?o ?g. }

• W2040809736 endingPage "446" @default.
• W2040809736 startingPage "435" @default.
• W2040809736 abstract "SMPI is a proteinaceous microbial metalloproteinase inhibitor that was isolated from Streptomyces nigrescens TK-23 in 1979. SMPI is known to selectively inhibit the metalloproteinases in the gluzincin family, according to the Rawling and Barrett classification. There has been no report on the interaction of a metalloproteinase in the family of gluzincins with its specific proteinaceous inhibitor. We have solved the solution structure of SMPI by NMR. Here, we report the binding mode of SMPI to thermolysin, based on the model complex structure generated using our high-resolution NMR structure of SMPI and the crystal structure of thermolysin. The obtained complex model shows that the extruded loop of SMPI, with the scissile bond Cys64-Val65, is complementary in shape to the active cleft of thermolysin. In the complex, the Cys64 (P1) carbonyl oxygen atom can form a tetrahedral coordination to the active zinc in thermolysin, and simultaneously, the methyl groups of Val65 (P1') are closely located in the hydrophobic S1' pocket in thermolysin. From the electrostatic potential surface calculation, the active loop of SMPI and the active cleft in thermolysin have been shown to be complementary in the surface charge distribution, resulting in the stabilization of the complex. The apparently large active loop is less flexible, but maintains a conformation in the nano- to picosecond time-scale, as elucidated from the 15N spin relaxation analysis. This is a quite different structural feature of SMPI from the flexible binding loop generally found in the serine proteinase inhibitors, such as SSI and eglin c, and can be related to the narrow specificity of SMPI. The present study provides the first insight into the interaction between a proteinaceous inhibitor and a gluzincin metalloproteinase." @default.
• W2040809736 created "2016-06-24" @default.
• W2040809736 creator A5018941669 @default.
• W2040809736 creator A5061174823 @default.
• W2040809736 creator A5063608760 @default.
• W2040809736 creator A5070577904 @default.
• W2040809736 creator A5076485810 @default.
• W2040809736 creator A5081784227 @default.
• W2040809736 date "1998-09-01" @default.
• W2040809736 modified "2023-10-18" @default.
• W2040809736 title "Elucidation of the mode of interaction of thermolysin with a proteinaceous metalloproteinase inhibitor, SMPI, based on a model complex structure and a structural dynamics analysis" @default.
• W2040809736 cites W104279188 @default.
• W2040809736 cites W1488354887 @default.
• W2040809736 cites W1558460354 @default.
• W2040809736 cites W1909498262 @default.
• W2040809736 cites W1928161708 @default.
• W2040809736 cites W1943721924 @default.
• W2040809736 cites W1965137801 @default.
• W2040809736 cites W1965724404 @default.
• W2040809736 cites W1968929239 @default.
• W2040809736 cites W1973057925 @default.
• W2040809736 cites W1982270821 @default.
• W2040809736 cites W1992437907 @default.
• W2040809736 cites W1997670079 @default.
• W2040809736 cites W2000125965 @default.
• W2040809736 cites W2000212022 @default.
• W2040809736 cites W2000314850 @default.
• W2040809736 cites W2000794695 @default.
• W2040809736 cites W2000903132 @default.
• W2040809736 cites W2002195659 @default.
• W2040809736 cites W2003424499 @default.
• W2040809736 cites W2005968596 @default.
• W2040809736 cites W2009168769 @default.
• W2040809736 cites W2012828649 @default.
• W2040809736 cites W2015004815 @default.
• W2040809736 cites W2021577368 @default.
• W2040809736 cites W2025717854 @default.
• W2040809736 cites W2026547695 @default.
• W2040809736 cites W2039132900 @default.
• W2040809736 cites W2047457611 @default.
• W2040809736 cites W2049115197 @default.
• W2040809736 cites W2050187607 @default.
• W2040809736 cites W2053360373 @default.
• W2040809736 cites W2053590485 @default.
• W2040809736 cites W2055337326 @default.
• W2040809736 cites W2063982283 @default.
• W2040809736 cites W2068227670 @default.
• W2040809736 cites W2070606233 @default.
• W2040809736 cites W2080717344 @default.
• W2040809736 cites W2082312460 @default.
• W2040809736 cites W2084459057 @default.
• W2040809736 cites W2086862822 @default.
• W2040809736 cites W2091977427 @default.
• W2040809736 cites W2093751310 @default.
• W2040809736 cites W2107353688 @default.
• W2040809736 cites W2120087635 @default.
• W2040809736 cites W2122563544 @default.
• W2040809736 cites W2137502578 @default.
• W2040809736 cites W2169821755 @default.
• W2040809736 cites W225911406 @default.
• W2040809736 cites W2339025468 @default.
• W2040809736 cites W2417252461 @default.
• W2040809736 cites W4242718594 @default.
• W2040809736 doi "https://doi.org/10.1006/jmbi.1998.2023" @default.
• W2040809736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9735298" @default.
• W2040809736 hasPublicationYear "1998" @default.
• W2040809736 type Work @default.
• W2040809736 sameAs 2040809736 @default.
• W2040809736 citedByCount "22" @default.
• W2040809736 countsByYear W20408097362015 @default.
• W2040809736 countsByYear W20408097362016 @default.
• W2040809736 crossrefType "journal-article" @default.
• W2040809736 hasAuthorship W2040809736A5018941669 @default.
• W2040809736 hasAuthorship W2040809736A5061174823 @default.
• W2040809736 hasAuthorship W2040809736A5063608760 @default.
• W2040809736 hasAuthorship W2040809736A5070577904 @default.
• W2040809736 hasAuthorship W2040809736A5076485810 @default.
• W2040809736 hasAuthorship W2040809736A5081784227 @default.
• W2040809736 hasConcept C181199279 @default.
• W2040809736 hasConcept C185020521 @default.
• W2040809736 hasConcept C185592680 @default.
• W2040809736 hasConcept C2780340462 @default.
• W2040809736 hasConcept C41183919 @default.
• W2040809736 hasConcept C55493867 @default.
• W2040809736 hasConcept C71240020 @default.
• W2040809736 hasConcept C87282627 @default.
• W2040809736 hasConceptScore W2040809736C181199279 @default.
• W2040809736 hasConceptScore W2040809736C185020521 @default.
• W2040809736 hasConceptScore W2040809736C185592680 @default.
• W2040809736 hasConceptScore W2040809736C2780340462 @default.
• W2040809736 hasConceptScore W2040809736C41183919 @default.
• W2040809736 hasConceptScore W2040809736C55493867 @default.
• W2040809736 hasConceptScore W2040809736C71240020 @default.
• W2040809736 hasConceptScore W2040809736C87282627 @default.
• W2040809736 hasFunder F4320320907 @default.
• W2040809736 hasFunder F4320321408 @default.
• W2040809736 hasFunder F4320338075 @default.
• W2040809736 hasIssue "2" @default.

• next