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- W2040832110 abstract "Molecular detection of HBV has a significant impact on prognosis and therapy of the disease. In this paper, a sensitive nucleic acid detection method of HBV was established taking advantage of magnetic nanoparticles (MNPs), chemiluminescence (CL) and polymerase chain reaction (PCR). HBV-DNA was extracted from hepatitis B positive human blood samples using MNPs adsorption method and biotin was labeled on the DNA segment after base insertion of bintin-dUTP in PCR. The biotinylated DNA segment was captured by amino probe immobilized on carboxyl MNPs and was detected by the chemiluminescence system of alkaline phosphatase catalyzing 3-(2'-spiroadamantane)-4-methoxy-4-(3-phosphoryloxy) phenyl-1, 2-dioxetane. Different concentrations of HBV-DNA were detected under the optimized experiment conditions and the relevant CL intensity were obtained, which provided a novel research or clinic diagnosis method for the quantification detection of HBV-DNA." @default.
- W2040832110 created "2016-06-24" @default.
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- W2040832110 date "2013-02-01" @default.
- W2040832110 modified "2023-10-05" @default.
- W2040832110 title "Chemiluminescence Analysis for HBV-DNA Hybridization Detection with Magnetic Nanoparticles Based DNA Extraction from Positive Whole Blood Samples" @default.
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- W2040832110 doi "https://doi.org/10.1166/jbn.2013.1478" @default.
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