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- W2040854967 abstract "Because superoxide is involved in various physiological processes, many efforts have been made to improve its accurate quantification. We optimized and validated a superoxide-specific and -sensitive detection method. The protocol is based on fluorescence detection of the superoxide-specific hydroethidine (HE) oxidation product, 2-hydroxyethidium. We established a method for the quantification of superoxide production in isolated mitochondria without the need for acetone extraction and purification chromatography as described in previous studies." @default.
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- W2040854967 date "2012-04-01" @default.
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- W2040854967 title "A simplified hydroethidine method for fast and accurate detection of superoxide production in isolated mitochondria" @default.
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- W2040854967 doi "https://doi.org/10.1016/j.ab.2012.01.008" @default.
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