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• W2040880212 abstract "Iodoacetamide and its spin-labeled derivative in high concentration ranges (10−2−10−1M) react with unexposed SH-groups of rhodopsin in the dark. The spin-labels bound to these SH-groups are moderately immobilized. While rhodopsin is not spin-labeled with the iodoacetamide at a lower concentration (5 × 10−4 m) in the dark, photobleached rhodopsin is readily spin-labeled with this concentration. The photo-bound spin-labels are highly immobilized and do not change their mobility after the regeneration of rhodopsin. Urea enhances the dark-bleaching of rhodopsin by iodoacetamide and increases the mobility of the bound spin-labels. The dark-reacting unexposed SH-groups appear to be different from the photo-exposed SH-groups which are not directly involved in the 500-nm chromophore. L'iodoacétamide et son dérivémarquéau spin réagissent aux fortes concentrations (10−2−10−1 m) dans l'obscuritéavec les groupes SH non exposes de la rhodopsine. Les spins marqués attachésàces groupes SH sont modérément immobilisés. Tandis que la rhodopsine n'a pas de spin marquépar l'iodoacétamide dans l'obscuritéàune faible concentration (5 × 10−4 m), la rhodopsine décolorée par la lumière est facilement marquée au spinàcette concentration. Les spins marqués accrochésàla lumière sont fortement immobilisés et ne changent pas de mobilitéaprès régénération de la rhodopsine. L'urée accroit la décoloration dans l'obscuritéde la rhodopsine par l'iodoacétamide et augmente la mobilitédes spins marqués attachés. Les groupes SH non exposés réagissantàl'obscuritésemblent différents des groupes SH photo-exposes qui ne sont pas directement actifs dans le chromophore de 500 nm. Jodazetamid und seine spin-tragenden Derivate reagieren in hoher Konzentration (10−2−10−1 m) im Dunkeln mit nicht exponierten SH-Gruppen von Rhodopsin. Die an die SH-Gruppen gebundenen spin-aktiven Gruppen sind massig unbeweglich. Während bei niedriger Konzentration (5 × 10−4M) im Dunkeln Rhodopsin durch Jodazetamid nicht spin-aktiviert wird, wird ausgebleichtes Rhodopsin auch bei dieser Konzentration leicht spin-aktiviert. Die bei Belichtung gebundenen spin-aktiven Gruppen sind sehr unbeweglich undändern ihre Beweglichkeit auch nicht nach der Regeneration des Rhodopsin. Urea verstärkt die Ausreichung von Rhodopsin im Dunkeln durch Jodazetamid und erhöht die Beweglichkeit der spin-aktiven Gruppen. Die im Dunkeln reagierenden nicht belichteten SH-Gruppen scheinen sich von den belichteten SH-Gruppen, die nicht direkt am 500 nm Chromophor beteiligt sind, zu unterscheiden. Иoдaцeтaмид и eгo cпиN-мeхeNNыe пpoизвoдNыe в oблacти выcoки кoNцeNтpaций (10−2−10−1 м) peaгиpyют c тpyдNoдocтyпNыми N-гpyппaми poдoпcиNa в тeмNoтe. CпиN-мeтки, cвязaNNыe c этими гpyппaми, yмepeNNo иммoбилизoвaNы. Xoтя poдoпcиN Ne cвязывaeтcя c иoдaцeтaмидoм пpи Nизких кoNцeNтpaциях (5 × 10−4 м) в тeмNoтe, фoтoвыцeтший poдoпcиN лeгкo мeтитcя пpи этих кoNцeNтpaциях. CпиN-мeтки, cвязaNNыe Na cвeтy, выcoкo иммoбилизoвaNы и Ne измeNяют cвoeй мoбильNocти пocлe peгeNepaции poдoпcиNa. MoхeвиNa ycиливaeт тeмNoвoe выцвeтaNиe poдoпcиNa иoдaцaцeтaмидoм и yвeлихивaeт мoбильNocть cвязaNNых cпиN-мeтoк. Peaгиpyющиe в тeмNoтe тpyдNoдocтyпNыe SH-гpyппы, пo-видимoмy, oтлихaютcя oт выcвoбoждaeмых Na cвeтy SH-гpyпп, кoтopыe Ne пpямo вклюхeNы в 500 Nм хpoмoфop." @default.
• W2040880212 created "2016-06-24" @default.
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• W2040880212 date "1975-01-01" @default.
• W2040880212 modified "2023-10-17" @default.
• W2040880212 title "Spin-labeled rhodopsin" @default.
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• W2040880212 doi "https://doi.org/10.1016/0042-6989(75)90060-7" @default.
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