FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040886323> ?p ?o ?g. }

• W2040886323 endingPage "56" @default.
• W2040886323 startingPage "45" @default.
• W2040886323 abstract "The extracellular domain of the PRL receptor (PRL-R) is composed of two subdomains of approximately 100 amino acids, S1 and S2. To explore the functional significance of these subdomains in PRL binding and signal transduction, deletion mutants of S1 or/and S2 subdomains were constructed. We report here the inability of each of these mutant receptor forms to bind PRL after expression in COS-7 cells. We also studied the abilities of these different mutant receptors to respond to hormonal stimulation after transfection of each mutant complementary DNA into CHO-K1 cells along with a chimeric gene containing the promoter of a milk protein gene (beta-lactoglobulin) fused to chloramphenicol acetyltransferase coding sequence. Somewhat unexpectedly, a constitutively (PRL-independent) mutant form of the PRL-R was obtained after deletion of the S2 subdomain. Moreover, we analyzed, in CHO-K1 cells, the biological activity of chimeric receptors constructs in which each subdomain sequence was replaced by an unrelated, but coding, sequence of foreign protein, and we confirmed a specific requirement for the S1 sequence in the constitutive activity. In contrast, the S2 subdomain produced an inhibitory effect on S1 constitutive activity. Cotransfection experiments with the wild-type receptor and the constitutive mutant receptor provided evidence that the wild-type receptor was able to inhibit the constitutive activity of the deleted mutant. Furthermore, in the mouse mammary epithelial cell line HC11, the constitutive PRL-R form was able to induce transcription of the beta-casein gene in the absence of PRL. These results suggest a complex signal transduction process that implicates each extracellular PRL-R subdomain. Possible mechanisms for the constitutive effect are discussed." @default.
• W2040886323 created "2016-06-24" @default.
• W2040886323 creator A5004102974 @default.
• W2040886323 creator A5009987807 @default.
• W2040886323 creator A5026808775 @default.
• W2040886323 creator A5028471780 @default.
• W2040886323 creator A5038074666 @default.
• W2040886323 creator A5064566803 @default.
• W2040886323 date "1996-01-01" @default.
• W2040886323 modified "2023-09-27" @default.
• W2040886323 title "Development of a constitutively active mutant form of the prolactin receptor, a member of the cytokine receptor family." @default.
• W2040886323 cites W132416300 @default.
• W2040886323 cites W1548111272 @default.
• W2040886323 cites W1593314637 @default.
• W2040886323 cites W160322612 @default.
• W2040886323 cites W1775749144 @default.
• W2040886323 cites W1942718916 @default.
• W2040886323 cites W1972927903 @default.
• W2040886323 cites W1974656027 @default.
• W2040886323 cites W1985832876 @default.
• W2040886323 cites W1989259214 @default.
• W2040886323 cites W1996548067 @default.
• W2040886323 cites W1997632886 @default.
• W2040886323 cites W2010356985 @default.
• W2040886323 cites W2012505338 @default.
• W2040886323 cites W2012527285 @default.
• W2040886323 cites W2018574052 @default.
• W2040886323 cites W2025864476 @default.
• W2040886323 cites W2030156849 @default.
• W2040886323 cites W2041190034 @default.
• W2040886323 cites W2047851489 @default.
• W2040886323 cites W2049208006 @default.
• W2040886323 cites W2058106399 @default.
• W2040886323 cites W2059460020 @default.
• W2040886323 cites W2060832558 @default.
• W2040886323 cites W2078913802 @default.
• W2040886323 cites W2084606144 @default.
• W2040886323 cites W2088622701 @default.
• W2040886323 cites W2100837269 @default.
• W2040886323 cites W2118929543 @default.
• W2040886323 cites W2134812217 @default.
• W2040886323 cites W2160600501 @default.
• W2040886323 cites W2435883539 @default.
• W2040886323 doi "https://doi.org/10.1210/mend.10.1.8838144" @default.
• W2040886323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8838144" @default.
• W2040886323 hasPublicationYear "1996" @default.
• W2040886323 type Work @default.
• W2040886323 sameAs 2040886323 @default.
• W2040886323 citedByCount "8" @default.
• W2040886323 countsByYear W20408863232013 @default.
• W2040886323 countsByYear W20408863232014 @default.
• W2040886323 crossrefType "journal-article" @default.
• W2040886323 hasAuthorship W2040886323A5004102974 @default.
• W2040886323 hasAuthorship W2040886323A5009987807 @default.
• W2040886323 hasAuthorship W2040886323A5026808775 @default.
• W2040886323 hasAuthorship W2040886323A5028471780 @default.
• W2040886323 hasAuthorship W2040886323A5038074666 @default.
• W2040886323 hasAuthorship W2040886323A5064566803 @default.
• W2040886323 hasBestOaLocation W20408863231 @default.
• W2040886323 hasConcept C104317684 @default.
• W2040886323 hasConcept C143065580 @default.
• W2040886323 hasConcept C150194340 @default.
• W2040886323 hasConcept C153911025 @default.
• W2040886323 hasConcept C161733203 @default.
• W2040886323 hasConcept C170493617 @default.
• W2040886323 hasConcept C17971392 @default.
• W2040886323 hasConcept C2779064019 @default.
• W2040886323 hasConcept C2781031661 @default.
• W2040886323 hasConcept C54009773 @default.
• W2040886323 hasConcept C55493867 @default.
• W2040886323 hasConcept C62478195 @default.
• W2040886323 hasConcept C71315377 @default.
• W2040886323 hasConcept C86803240 @default.
• W2040886323 hasConcept C95444343 @default.
• W2040886323 hasConceptScore W2040886323C104317684 @default.
• W2040886323 hasConceptScore W2040886323C143065580 @default.
• W2040886323 hasConceptScore W2040886323C150194340 @default.
• W2040886323 hasConceptScore W2040886323C153911025 @default.
• W2040886323 hasConceptScore W2040886323C161733203 @default.
• W2040886323 hasConceptScore W2040886323C170493617 @default.
• W2040886323 hasConceptScore W2040886323C17971392 @default.
• W2040886323 hasConceptScore W2040886323C2779064019 @default.
• W2040886323 hasConceptScore W2040886323C2781031661 @default.
• W2040886323 hasConceptScore W2040886323C54009773 @default.
• W2040886323 hasConceptScore W2040886323C55493867 @default.
• W2040886323 hasConceptScore W2040886323C62478195 @default.
• W2040886323 hasConceptScore W2040886323C71315377 @default.
• W2040886323 hasConceptScore W2040886323C86803240 @default.
• W2040886323 hasConceptScore W2040886323C95444343 @default.
• W2040886323 hasIssue "1" @default.
• W2040886323 hasLocation W20408863231 @default.
• W2040886323 hasLocation W20408863232 @default.
• W2040886323 hasLocation W20408863233 @default.
• W2040886323 hasOpenAccess W2040886323 @default.
• W2040886323 hasPrimaryLocation W20408863231 @default.
• W2040886323 hasRelatedWork W2002878567 @default.
• W2040886323 hasRelatedWork W2009464217 @default.
• W2040886323 hasRelatedWork W2011381989 @default.

• next