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• W2040886476 abstract "We have examined the pharmacological subtypes of muscarinic receptors mediating phosphoinositide hydrolysis and contraction in the longitudinal smooth muscle of guinea pig ileum with the use of muscarinic antagonists. Carbachol increased the formation of 3 H-inositol phosphates (IPs) in a dose-dependent manner in both ileal smooth muscle and the frontal cortex of rats. The rank order of muscarinic antagonists for inhibition of IP formation induced by carbachol was 4-DAMP = atropine > pirenzepine > AF-DX 116 in guinea pig ileal smooth muscle. In ileal smooth muscle, the inhibition by the M 1 antagonist pirenzepine was about 15 times less than that by atropine. However, in the rat frontal cortex, the inhibition by pirenzepine was only about 3 times less than that by atropine. The inhibitory effect of the M 2 antagonist AF-DX 116 was weak in both ileal muscle and the frontal cortex. The M 3 antagonist 4-DAMP strongly inhibited carbachol-induced IP formation in ileal smooth muscle. The rank order of muscarinic antagonists for inhibition of contraction induced by 10 7 M carbachol was atropine ≥ 4-DAMP > pirenzepine > AF-DX 116. These results suggest that both IP formation and the contractile response induced by muscarinic agonists are mediated through the muscarinic M 3 subtype in guinea pig ileum." @default.
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• W2040886476 date "1993-01-01" @default.
• W2040886476 modified "2023-10-16" @default.
• W2040886476 title "Pharmacological Profiles of Muscarinic Receptors in the Longitudinal Smooth Muscle of Guinea Pig Ileum" @default.
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• W2040886476 doi "https://doi.org/10.1254/jjp.62.257" @default.
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