FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040896508> ?p ?o ?g. }

• W2040896508 endingPage "294" @default.
• W2040896508 startingPage "289" @default.
• W2040896508 abstract "Ficolin is characterized by the presence of both collagen-like and fibrinogen-like sequences, and potentially has a similar overall structure as the complement protein C1q and the collectins. Previous studies have reported the presence of human ficolin mRNA predominantly in peripheral blood leucocytes. In the present study, the cellular origin of human ficolin was investigated in further detail. Preliminary studies using reverse transcriptase–polymerase chain reaction (RT–PCR) showed that ficolin mRNA was synthesized by U937 cells, a human monocyte cell line. This finding suggested that blood monocytes also normally synthesize human ficolin. Peripheral blood monocytes from adult human donors were harvested at serial time-points (0–20 hr) after adhesion to tissue culture plates, and total RNA was isolated and assayed for ficolin mRNA by RT–PCR. Ficolin mRNA was highly expressed in monocytes throughout the first 20 hr of adhesion. In contrast, C1q and mannose receptor mRNA were not detectable during the first 8 hr of adhesion, but were highly expressed by 20 hr. Cells were harvested at longer time intervals (1, 2, 4, 6 and 8 days) to determine whether ficolin expression was temporally regulated at later stages of monocyte differentiation. Ficolin mRNA levels decreased sharply from day 1 to day 6. In contrast, the levels of both C1q and mannose receptor mRNA showed no changing trend. These results are consistent with the absence of ficolin expression in many macrophage-rich tissues previously reported. The origin of ficolin from monocytes, together with its structural similarity to C1q and the collectins, raises the possibility that ficolin may be another plasma protein capable of binding to surface structures of micro-organisms. Escherichia coli was therefore incubated with human serum, and bound proteins, after elution with sugars, were analysed by Western blotting using an antiserum raised against a synthetic ficolin peptide. The antiserum identified a polypeptide of approximately 42 000 MW, which is similar in size to that of ficolin as predicted from its cDNA-derived sequence." @default.
• W2040896508 created "2016-06-24" @default.
• W2040896508 creator A5009905635 @default.
• W2040896508 creator A5022308603 @default.
• W2040896508 creator A5032706436 @default.
• W2040896508 creator A5044142602 @default.
• W2040896508 creator A5072581113 @default.
• W2040896508 date "1996-10-01" @default.
• W2040896508 modified "2023-09-27" @default.
• W2040896508 title "Biosynthesis of human ficolin, an Escherichia coli-binding protein, by monocytes: comparison with the synthesis of two macrophage-specific proteins, C1q and the mannose receptor" @default.
• W2040896508 cites W1571263856 @default.
• W2040896508 cites W1588226764 @default.
• W2040896508 cites W1820570900 @default.
• W2040896508 cites W1980427216 @default.
• W2040896508 cites W1981534559 @default.
• W2040896508 cites W1986055788 @default.
• W2040896508 cites W2047774312 @default.
• W2040896508 cites W2059882274 @default.
• W2040896508 cites W2082183799 @default.
• W2040896508 cites W2134812217 @default.
• W2040896508 cites W2151508727 @default.
• W2040896508 cites W2405090893 @default.
• W2040896508 cites W2408204569 @default.
• W2040896508 cites W4232157339 @default.
• W2040896508 cites W98597308 @default.
• W2040896508 doi "https://doi.org/10.1046/j.1365-2567.1996.d01-732.x" @default.
• W2040896508 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1456477" @default.
• W2040896508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8943728" @default.
• W2040896508 hasPublicationYear "1996" @default.
• W2040896508 type Work @default.
• W2040896508 sameAs 2040896508 @default.
• W2040896508 citedByCount "79" @default.
• W2040896508 countsByYear W20408965082012 @default.
• W2040896508 countsByYear W20408965082013 @default.
• W2040896508 countsByYear W20408965082014 @default.
• W2040896508 countsByYear W20408965082015 @default.
• W2040896508 countsByYear W20408965082016 @default.
• W2040896508 countsByYear W20408965082017 @default.
• W2040896508 countsByYear W20408965082018 @default.
• W2040896508 countsByYear W20408965082019 @default.
• W2040896508 countsByYear W20408965082020 @default.
• W2040896508 countsByYear W20408965082021 @default.
• W2040896508 countsByYear W20408965082022 @default.
• W2040896508 crossrefType "journal-article" @default.
• W2040896508 hasAuthorship W2040896508A5009905635 @default.
• W2040896508 hasAuthorship W2040896508A5022308603 @default.
• W2040896508 hasAuthorship W2040896508A5032706436 @default.
• W2040896508 hasAuthorship W2040896508A5044142602 @default.
• W2040896508 hasAuthorship W2040896508A5072581113 @default.
• W2040896508 hasBestOaLocation W20408965082 @default.
• W2040896508 hasConcept C104317684 @default.
• W2040896508 hasConcept C105580179 @default.
• W2040896508 hasConcept C124594742 @default.
• W2040896508 hasConcept C133493029 @default.
• W2040896508 hasConcept C136449434 @default.
• W2040896508 hasConcept C153911025 @default.
• W2040896508 hasConcept C170493617 @default.
• W2040896508 hasConcept C189413060 @default.
• W2040896508 hasConcept C202751555 @default.
• W2040896508 hasConcept C203014093 @default.
• W2040896508 hasConcept C2775887612 @default.
• W2040896508 hasConcept C2776205754 @default.
• W2040896508 hasConcept C2779244956 @default.
• W2040896508 hasConcept C2780758401 @default.
• W2040896508 hasConcept C2781184567 @default.
• W2040896508 hasConcept C55493867 @default.
• W2040896508 hasConcept C86803240 @default.
• W2040896508 hasConceptScore W2040896508C104317684 @default.
• W2040896508 hasConceptScore W2040896508C105580179 @default.
• W2040896508 hasConceptScore W2040896508C124594742 @default.
• W2040896508 hasConceptScore W2040896508C133493029 @default.
• W2040896508 hasConceptScore W2040896508C136449434 @default.
• W2040896508 hasConceptScore W2040896508C153911025 @default.
• W2040896508 hasConceptScore W2040896508C170493617 @default.
• W2040896508 hasConceptScore W2040896508C189413060 @default.
• W2040896508 hasConceptScore W2040896508C202751555 @default.
• W2040896508 hasConceptScore W2040896508C203014093 @default.
• W2040896508 hasConceptScore W2040896508C2775887612 @default.
• W2040896508 hasConceptScore W2040896508C2776205754 @default.
• W2040896508 hasConceptScore W2040896508C2779244956 @default.
• W2040896508 hasConceptScore W2040896508C2780758401 @default.
• W2040896508 hasConceptScore W2040896508C2781184567 @default.
• W2040896508 hasConceptScore W2040896508C55493867 @default.
• W2040896508 hasConceptScore W2040896508C86803240 @default.
• W2040896508 hasIssue "2" @default.
• W2040896508 hasLocation W20408965081 @default.
• W2040896508 hasLocation W20408965082 @default.
• W2040896508 hasLocation W20408965083 @default.
• W2040896508 hasOpenAccess W2040896508 @default.
• W2040896508 hasPrimaryLocation W20408965081 @default.
• W2040896508 hasRelatedWork W1964144818 @default.
• W2040896508 hasRelatedWork W1994366741 @default.
• W2040896508 hasRelatedWork W2006581388 @default.
• W2040896508 hasRelatedWork W2007760924 @default.
• W2040896508 hasRelatedWork W2018152003 @default.
• W2040896508 hasRelatedWork W2065178571 @default.
• W2040896508 hasRelatedWork W2121327910 @default.
• W2040896508 hasRelatedWork W2130803238 @default.

• next