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- W2040916455 abstract "Most studies on DNA-based immunization have used viral promoters to drive antigen expression. Recently, the use of tissue-specific DNA vaccines has been favored regarding safety issues. In this study, we determined the impact of antigen localization and tissue-specific expression on the induction of humoral as well as cellular immune responses in a BALB/c mouse model. Thereby, we show that using the muscle-specific muscle creatine kinase (MCK) promoter/enhancer the efficiency of immune stimulation is strictly dependent on the ability of HIV-1 Pr55gag to be released from cells. By contrast, localization of Pr55gag and derivatives thereof plays only a minor role when antigen is constitutively expressed using the ubiquitous viral CMV promoter." @default.
- W2040916455 created "2016-06-24" @default.
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- W2040916455 date "2002-05-01" @default.
- W2040916455 modified "2023-09-25" @default.
- W2040916455 title "Efficiency of a myogenic DNA vaccine is strictly dependent upon cellular localization of HIV-1 Pr55gag" @default.
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- W2040916455 doi "https://doi.org/10.1016/s0264-410x(02)00082-8" @default.
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