Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040962073> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2040962073 endingPage "374" @default.
- W2040962073 startingPage "368" @default.
- W2040962073 abstract "The conditions for phosphatidylethanolamine (PE)-diaglycerol (DAG) exchange catalysed by cell-free extracts of Escherichia coli were studied using 14C- or 3H-analogues of both these lipids. The reaction, examined with either labelled PE or labelled DAG, occurred without co-factor addition and was inhibited by Ca2+ and Mg2+. Detergents such as Triton X-100 greatly enhanced the activity; however, the optimal concentration of this agent depended on the lipid substrate concentration. The exchange-catalysing enzyme involved in these extracts appeared to very specific for DAG and PE, since no other labelled phospholipid or acylglycerol derivative formed radioactive product under the assay conditions tested. Again, endogenous [3H]PE present in the enzyme source, but no other endogenous lipid, was converted to labelled DAG in the presence of added 1,2-dioleoyl-sn-glycerol. The Vmax value for the conversion of labelled PE to DAG was very similar to the Vmax value found for the conversion of labelled DAG to PE as would be expected in the case of an exchange reaction being responsible for both conversions. However, the Km value for PE was appreciably larger than that for DAG. The enzyme involved, displayed a broad acyl chain specificity as could be judged from: (1) the ability of various species of DAG and PE to stimulate the exchange; (2) the suitability of lipid substrates prepared from widely different biological sources; and (3) the interchange of acyl groups that occurred between dimyristoyl PE and dilauroylglycerol. As would be expected for an exchange reaction, the incorporation of lauroyl groups into PE occurred without an increase in the total fatty acid content of this phospholipid. The results of the present study confirm and further characterize the PE-DAG exchange reaction of E. coli." @default.
- W2040962073 created "2016-06-24" @default.
- W2040962073 creator A5044629478 @default.
- W2040962073 creator A5088766675 @default.
- W2040962073 date "1990-06-01" @default.
- W2040962073 modified "2023-09-27" @default.
- W2040962073 title "Further characterization of the diacylglycerol-phosphatidyllethanolamine exchange reaction catalyzed by cell-free extracts of Escherichia coli" @default.
- W2040962073 cites W1548518275 @default.
- W2040962073 cites W1612525643 @default.
- W2040962073 cites W1775749144 @default.
- W2040962073 cites W1996899694 @default.
- W2040962073 cites W2010750968 @default.
- W2040962073 cites W2019847905 @default.
- W2040962073 cites W2048401373 @default.
- W2040962073 cites W2138704472 @default.
- W2040962073 cites W2152311269 @default.
- W2040962073 cites W2168091367 @default.
- W2040962073 cites W4248672912 @default.
- W2040962073 doi "https://doi.org/10.1016/0005-2760(90)90082-9" @default.
- W2040962073 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2194574" @default.
- W2040962073 hasPublicationYear "1990" @default.
- W2040962073 type Work @default.
- W2040962073 sameAs 2040962073 @default.
- W2040962073 citedByCount "0" @default.
- W2040962073 crossrefType "journal-article" @default.
- W2040962073 hasAuthorship W2040962073A5044629478 @default.
- W2040962073 hasAuthorship W2040962073A5088766675 @default.
- W2040962073 hasConcept C104317684 @default.
- W2040962073 hasConcept C161790260 @default.
- W2040962073 hasConcept C181199279 @default.
- W2040962073 hasConcept C185592680 @default.
- W2040962073 hasConcept C18903297 @default.
- W2040962073 hasConcept C193557335 @default.
- W2040962073 hasConcept C195794163 @default.
- W2040962073 hasConcept C198684833 @default.
- W2040962073 hasConcept C2776330855 @default.
- W2040962073 hasConcept C2777289219 @default.
- W2040962073 hasConcept C2778918659 @default.
- W2040962073 hasConcept C2780881558 @default.
- W2040962073 hasConcept C2781170229 @default.
- W2040962073 hasConcept C36020004 @default.
- W2040962073 hasConcept C41625074 @default.
- W2040962073 hasConcept C43617362 @default.
- W2040962073 hasConcept C547475151 @default.
- W2040962073 hasConcept C55493867 @default.
- W2040962073 hasConcept C86803240 @default.
- W2040962073 hasConceptScore W2040962073C104317684 @default.
- W2040962073 hasConceptScore W2040962073C161790260 @default.
- W2040962073 hasConceptScore W2040962073C181199279 @default.
- W2040962073 hasConceptScore W2040962073C185592680 @default.
- W2040962073 hasConceptScore W2040962073C18903297 @default.
- W2040962073 hasConceptScore W2040962073C193557335 @default.
- W2040962073 hasConceptScore W2040962073C195794163 @default.
- W2040962073 hasConceptScore W2040962073C198684833 @default.
- W2040962073 hasConceptScore W2040962073C2776330855 @default.
- W2040962073 hasConceptScore W2040962073C2777289219 @default.
- W2040962073 hasConceptScore W2040962073C2778918659 @default.
- W2040962073 hasConceptScore W2040962073C2780881558 @default.
- W2040962073 hasConceptScore W2040962073C2781170229 @default.
- W2040962073 hasConceptScore W2040962073C36020004 @default.
- W2040962073 hasConceptScore W2040962073C41625074 @default.
- W2040962073 hasConceptScore W2040962073C43617362 @default.
- W2040962073 hasConceptScore W2040962073C547475151 @default.
- W2040962073 hasConceptScore W2040962073C55493867 @default.
- W2040962073 hasConceptScore W2040962073C86803240 @default.
- W2040962073 hasIssue "3" @default.
- W2040962073 hasLocation W20409620731 @default.
- W2040962073 hasLocation W20409620732 @default.
- W2040962073 hasOpenAccess W2040962073 @default.
- W2040962073 hasPrimaryLocation W20409620731 @default.
- W2040962073 hasRelatedWork W1541958084 @default.
- W2040962073 hasRelatedWork W1769442777 @default.
- W2040962073 hasRelatedWork W1972665833 @default.
- W2040962073 hasRelatedWork W1982477616 @default.
- W2040962073 hasRelatedWork W1995146598 @default.
- W2040962073 hasRelatedWork W2012570415 @default.
- W2040962073 hasRelatedWork W2082730917 @default.
- W2040962073 hasRelatedWork W2130723068 @default.
- W2040962073 hasRelatedWork W2747434649 @default.
- W2040962073 hasRelatedWork W1712896731 @default.
- W2040962073 hasVolume "1044" @default.
- W2040962073 isParatext "false" @default.
- W2040962073 isRetracted "false" @default.
- W2040962073 magId "2040962073" @default.
- W2040962073 workType "article" @default.