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• W2040962905 startingPage "447" @default.
• W2040962905 abstract "Background: Tea tree oil is an aboriginal Australian traditional medicine for bruises, insect bites, and skin infections. It was redis-covered in the 1920s as a topical antiseptic that is more effective than Phenol. Previous studies have demonstrated its antiseptic qual-ities, but its effects on human white blood cells have never been investigated. Objective: To test the hypothesis that tea tree oil exerts its antiseptic action through white blood cell activation. Methods: Crude oil and the purified “active” component were studied by using a model system that responds to bioactive components by induction of differentiation in white blood cells. Methods used included white blood cell oxidative burst assay (nitroblue tetrazolium [NBT] dye reduction); cell prolif-eration assay (tritiated thymidine incorporation); cell surface differentiation marker assay (flow cytometric quantitation of phycoerythrin-anti-CD 11b binding); cell via-bility assay (trypan blue exclusion); and cellu-lar differentiation enzyme assay (white cell esterase staining). Results: Collectively, five assays that measure differentiation in white blood cells indicated monocytic differentiation after treatment with either crude oil or the purified active component. Both the crude oil and the purified active component, (+:−) terpinene-4-ol, caused a similar type and amount of differentiation. The cul-ture of cells in medium containing serum caused more activation than in medium containing no serum. Conclusion: The antiseptic activity of tea tree oil appears to be due, in part, to white blood cell activation. (J Manipulative Physiol Ther 1999;22:447–53)" @default.
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• W2040962905 date "1999-09-01" @default.
• W2040962905 modified "2023-09-27" @default.
• W2040962905 title "Biological activity of Melaleuca alternifolia (tea tree) oil component, terpinen-4-ol, in human myelocytic cell line HL-60" @default.
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• W2040962905 doi "https://doi.org/10.1016/s0161-4754(99)70033-3" @default.
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