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- W2040982193 abstract "The detection of atrazine using a novel optical immunosensing technique based on negative dielectrophoresis (n-DEP) in microfluidic channels is described. Atrazine is a toxic triazine herbicide within the most frequently used. Polystyrene microparticles (6 μm diameters) modified with bovine serum albumin conjugated with atrazine (atrazine-BSA) were manipulated and captured when subjected to intense n-DEP electric fields. Specifically, particles were trapped when AC voltages with amplitudes of 10 Vpeak and frequencies over 1 MHz were applied to the electrodes. The immunological reaction occurring on the particles for detecting atrazine is based on an indirect competitive assay using a secondary anti-mouse immunogloburin G (IgG) antibody labeled with fluorescein isothiocyanate. The microfluidic device, with three-dimensional microelectrodes, was fabricated comprising two caged areas, allowing two simultaneous measurements inside the same microfluidic channel. The performance of this n-DEP immunosensing technique was evaluated using wine samples. The immunodevice showed a limit of detection for atrazine in buffer samples of 0.11 μg L−1 and in pre-treated wine samples of 6.8 μg L−1; these detection limits are lower than the maximum residue level (MRL) established by the European Community for residues of this herbicide in wine (50 μg L−1). This methodology offers great promise for rapid, simple, cost effective, and on-site analysis of biological, foods and beverages, and environmental samples." @default.
- W2040982193 created "2016-06-24" @default.
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- W2040982193 date "2009-02-01" @default.
- W2040982193 modified "2023-10-18" @default.
- W2040982193 title "Detection of pesticide residues using an immunodevice based on negative dielectrophoresis" @default.
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- W2040982193 doi "https://doi.org/10.1016/j.bios.2008.08.035" @default.
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