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• W2040982827 abstract "Recently, the immunodiagnostic tests, interferon-γ (IFN-γ) release assays (IGRAs) for TB infection, have been developed. These tests are based on the Mycobacterium tuberculosis specific antigens early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10), and include a whole-blood interferon-γ enzyme linked immunosorbent assay (QuantiFERON-TB Gold [QFT-G], Cellestis Ltd, Victoria, Australia) and an enzyme-linked immunospot assay (T SPOT.TB, Oxford Immunotec, Oxfordshire, UK). Both tests have shown promising results in the detection of latent TB infection (LTBI) 1 Menzies D. Pai M. Comstock G. Meta-analysis: new tests for the diagnosis of latent tuberculosis infection: areas of uncertainty and recommendations for research. Ann Intern Med. 2007; 146: 340-354 Crossref PubMed Scopus (879) Google Scholar and the QFT-G is a Food and Drug Administration (FDA) U.S. approved and Centers for Disease Control and Prevention (CDC) recommended test for diagnosing latent tuberculosis. 2 Mazurek G.H. Jereb J. Lobue P. Iademarco M.F. Metchock B. Vernon A. Guideline for using QuantiFERON-TB gold test for detecting Mycobacterium tuberculosis infection, United States. Morb Mortal Wkly Rep. 2005; 54: 49-55 Google Scholar Furthermore, several studies demonstrated that Interferon-γ release assays maybe a useful tool in the diagnosis of active tuberculosis. 3 Kang Y.A. Lee H.W. Hwang S.S. Um S.W. Han S.K. Shim Y.S. et al. Usefulness of whole-blood interferon-gamma assay and interferon-gamma enzyme-linked immunospot assay in the diagnosis of active pulmonary tuberculosis. Chest. 2007; 132: 959-965 Crossref PubMed Scopus (118) Google Scholar , 4 Lai C.C. Liao C.H. Tan C.K. Chou C.H. Huang Y.T. Hsueh P.R. Diagnosis of peripheral tuberculous lymphadenitis by enzyme-linked immunospot assay for interferon-gamma. Am J Med. 2009; 122: e3 Abstract Full Text Full Text PDF PubMed Scopus (18) Google Scholar , 5 Lai C.C. Lee T.C. Hsiao C.H. Liao C.H. Chou C.H. Tan C.K. et al. Differential diagnosis of Crohn's disease and intestinal tuberculous by enzyme-linked immunospot assay for interferon-γ. Am J Gastroenterol. 2009; 104: 2121-2122 Crossref PubMed Scopus (14) Google Scholar However, its specificity and sensitivity in this area varies among the different study populations and different methods. 3 Kang Y.A. Lee H.W. Hwang S.S. Um S.W. Han S.K. Shim Y.S. et al. Usefulness of whole-blood interferon-gamma assay and interferon-gamma enzyme-linked immunospot assay in the diagnosis of active pulmonary tuberculosis. Chest. 2007; 132: 959-965 Crossref PubMed Scopus (118) Google Scholar , 4 Lai C.C. Liao C.H. Tan C.K. Chou C.H. Huang Y.T. Hsueh P.R. Diagnosis of peripheral tuberculous lymphadenitis by enzyme-linked immunospot assay for interferon-gamma. Am J Med. 2009; 122: e3 Abstract Full Text Full Text PDF PubMed Scopus (18) Google Scholar , 5 Lai C.C. Lee T.C. Hsiao C.H. Liao C.H. Chou C.H. Tan C.K. et al. Differential diagnosis of Crohn's disease and intestinal tuberculous by enzyme-linked immunospot assay for interferon-γ. Am J Gastroenterol. 2009; 104: 2121-2122 Crossref PubMed Scopus (14) Google Scholar , 6 Dheda K. Pooran A. Pai M. Miller R.F. Lesley K. Booth H.L. et al. Interpretation of Mycobacterium tuberculosis antigen-specific IFN-gamma release assays (T-SPOT.TB) and factors that modulate test results. J Infect. 2007; 55: 169-173 Abstract Full Text Full Text PDF PubMed Scopus (55) Google Scholar , 7 Harada N. Higuchi K. Yoshiyama T. Kawabe Y. Fujita A. Sasaki Y. et al. Comparison of the sensitivity and specificity of two whole blood interferon-gamma assays for M. tuberculosis infection. J Infect. 2008; 56: 348-353 Abstract Full Text Full Text PDF PubMed Scopus (106) Google Scholar Despite the connection between false negative IFN-γ release assay results and age above 60 years, female sex and CD4 cell counts have been described in HIV-infected patients, 8 Rangaka M.X. Wilkinson K.A. Seldon R. Van Cutsem G. Meintjes G.A. Morroni C. et al. Effect of HIV-1 infection on T-cell-based and skin test detection of tuberculosis infection. Am J Respir Crit Care Med. 2007; 175: 514-520 Crossref PubMed Scopus (195) Google Scholar , 9 Raby E. Moyo M. Devendra A. Banda J. De Haas P. Ayles H. et al. The effects of HIV on the sensitivity of a whole blood IFN-gamma release assay in Zambian adults with active tuberculosis. PLoS ONE. 2008; 3: e2489 Crossref PubMed Scopus (109) Google Scholar , 10 Ak O. Dabak G. Ozer S. Saygi A. Dabak R. The evaluation of the quantiferon-TB gold test in pulmonary and extrapulmonary tuberculosis. Jpn J Infect Dis. 2009; 62: 149-151 PubMed Google Scholar , 11 Dewan P.K. Ginsdale J. Kawamura M. Low sensitivity of a whole-blood interferon-γ assay for detection of active tuberculosis. Clin Infect Dis. 2007; 44: 69-73 Crossref PubMed Scopus (125) Google Scholar the knowledge about the association with false negative ELISPOT assays among non-HIV-infected patients with various underlying diseases is limited." @default.
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• W2040982827 title "False-negative results by enzyme-linked immunospot assay for interferon-γ among patients with culture-confirmed tuberculosis" @default.
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