FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040992047> ?p ?o ?g. }

• W2040992047 endingPage "1256" @default.
• W2040992047 startingPage "1246" @default.
• W2040992047 abstract "Myofibroblasts derived from portal fibroblasts are important fibrogenic cells in the early stages of biliary fibrosis. In contrast to hepatic stellate cells, portal fibroblasts have not been well studied in vitro, and little is known about their myofibroblastic differentiation. In this article we report the isolation and characterization of rat portal fibroblasts in culture. We demonstrate that primary portal fibroblasts undergo differentiation to α-smooth muscle actin–expressing myofibroblasts over 10–14 days. Marker analysis comparing portal fibroblasts to hepatic stellate cells demonstrated that these are distinct populations and that staining with elastin and desmin can differentiate between them. Portal fibroblasts expressed elastin at all stages in culture but never expressed desmin, whereas hepatic stellate cells consistently expressed desmin but never elastin. Immunostaining of rat liver tissue confirmed these results in vivo. Characterization of portal fibroblast differentiation in culture demonstrated that these cells required transforming growth factor-β (TGF-β): cells remained quiescent in the presence of a TGF-β receptor kinase inhibitor, whereas exogenous TGF-β1 enhanced portal fibroblast α-smooth muscle actin expression and stress fiber formation. In contrast, platelet-derived growth factor inhibited myofibroblastic differentiation. Portal fibroblasts were also dependent on mechanical tension for myofibroblastic differentiation, and cells cultured on polyacrylamide supports of variable stiffness demonstrated an increasingly myofibroblastic phenotype as stiffness increased. Conclusion: Portal fibroblasts are morphologically and functionally distinct from hepatic stellate cells. Portal fibroblast myofibroblastic differentiation can be modeled in culture and requires both TGF-β and mechanical tension. (HEPATOLOGY 2007.)" @default.
• W2040992047 created "2016-06-24" @default.
• W2040992047 creator A5009962061 @default.
• W2040992047 creator A5012615383 @default.
• W2040992047 creator A5033862323 @default.
• W2040992047 creator A5045288947 @default.
• W2040992047 creator A5056572795 @default.
• W2040992047 creator A5058119915 @default.
• W2040992047 date "2007-10-01" @default.
• W2040992047 modified "2023-10-18" @default.
• W2040992047 title "Transforming growth factor-β and substrate stiffness regulate portal fibroblast activation in culture" @default.
• W2040992047 cites W1971753613 @default.
• W2040992047 cites W1973089587 @default.
• W2040992047 cites W1973694149 @default.
• W2040992047 cites W1977815598 @default.
• W2040992047 cites W1980394389 @default.
• W2040992047 cites W1982130586 @default.
• W2040992047 cites W1987291131 @default.
• W2040992047 cites W1993518161 @default.
• W2040992047 cites W1993891700 @default.
• W2040992047 cites W1996383717 @default.
• W2040992047 cites W2000581973 @default.
• W2040992047 cites W2002582091 @default.
• W2040992047 cites W2009986655 @default.
• W2040992047 cites W2013099264 @default.
• W2040992047 cites W2020600880 @default.
• W2040992047 cites W2025294090 @default.
• W2040992047 cites W2028886123 @default.
• W2040992047 cites W2032966552 @default.
• W2040992047 cites W2033688149 @default.
• W2040992047 cites W2039876496 @default.
• W2040992047 cites W2045735841 @default.
• W2040992047 cites W2046636382 @default.
• W2040992047 cites W2048093238 @default.
• W2040992047 cites W2050495493 @default.
• W2040992047 cites W2054635074 @default.
• W2040992047 cites W2057698117 @default.
• W2040992047 cites W2058768743 @default.
• W2040992047 cites W2063307081 @default.
• W2040992047 cites W2065757450 @default.
• W2040992047 cites W2077930885 @default.
• W2040992047 cites W2080325240 @default.
• W2040992047 cites W2088724129 @default.
• W2040992047 cites W2095301611 @default.
• W2040992047 cites W2099071997 @default.
• W2040992047 cites W2118858353 @default.
• W2040992047 cites W2126232334 @default.
• W2040992047 cites W2134601540 @default.
• W2040992047 cites W2139327890 @default.
• W2040992047 cites W2148328620 @default.
• W2040992047 cites W2154807626 @default.
• W2040992047 cites W2162099037 @default.
• W2040992047 cites W2179353943 @default.
• W2040992047 cites W2188009983 @default.
• W2040992047 cites W4255712601 @default.
• W2040992047 doi "https://doi.org/10.1002/hep.21792" @default.
• W2040992047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17625791" @default.
• W2040992047 hasPublicationYear "2007" @default.
• W2040992047 type Work @default.
• W2040992047 sameAs 2040992047 @default.
• W2040992047 citedByCount "302" @default.
• W2040992047 countsByYear W20409920472012 @default.
• W2040992047 countsByYear W20409920472013 @default.
• W2040992047 countsByYear W20409920472014 @default.
• W2040992047 countsByYear W20409920472015 @default.
• W2040992047 countsByYear W20409920472016 @default.
• W2040992047 countsByYear W20409920472017 @default.
• W2040992047 countsByYear W20409920472018 @default.
• W2040992047 countsByYear W20409920472019 @default.
• W2040992047 countsByYear W20409920472020 @default.
• W2040992047 countsByYear W20409920472021 @default.
• W2040992047 countsByYear W20409920472022 @default.
• W2040992047 countsByYear W20409920472023 @default.
• W2040992047 crossrefType "journal-article" @default.
• W2040992047 hasAuthorship W2040992047A5009962061 @default.
• W2040992047 hasAuthorship W2040992047A5012615383 @default.
• W2040992047 hasAuthorship W2040992047A5033862323 @default.
• W2040992047 hasAuthorship W2040992047A5045288947 @default.
• W2040992047 hasAuthorship W2040992047A5056572795 @default.
• W2040992047 hasAuthorship W2040992047A5058119915 @default.
• W2040992047 hasConcept C104317684 @default.
• W2040992047 hasConcept C134018914 @default.
• W2040992047 hasConcept C142724271 @default.
• W2040992047 hasConcept C147447768 @default.
• W2040992047 hasConcept C148738053 @default.
• W2040992047 hasConcept C153911025 @default.
• W2040992047 hasConcept C176891718 @default.
• W2040992047 hasConcept C203014093 @default.
• W2040992047 hasConcept C204232928 @default.
• W2040992047 hasConcept C207865475 @default.
• W2040992047 hasConcept C2779072444 @default.
• W2040992047 hasConcept C2780381497 @default.
• W2040992047 hasConcept C2780559512 @default.
• W2040992047 hasConcept C54355233 @default.
• W2040992047 hasConcept C55493867 @default.
• W2040992047 hasConcept C71924100 @default.
• W2040992047 hasConcept C81885089 @default.
• W2040992047 hasConcept C86803240 @default.

• next