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• W2040994414 abstract "Low doses of progestogens in regimens for synchronization of estrus result in persistent follicles (PF) and reduced conception rates in cattle. The effects of PF on recovery of oocytes/embryos, fertilization, and embryonic development were examined in beef cows. To produce PF, cows received a used Controlled Internal Drug Releasing device on Days 4 through 13 after pretreatment estrus (Day 0) and two injections of 20 mg prostaglandin (PG) F2α, i.m., 12 h apart on Day 6 (PF; n = 20). Control cows received only PGF2α on Day 6 so they would ovulate growing follicles (GF; n = 22). With this model, the largest (dominant) follicle from the first wave of follicular development was being ovulated in cows in each group. Cows were observed for estrus twice daily and were then mated to highly fertile bulls. Follicular development was monitored by ultrasonography on alternate days from Day 2 until estrus and then daily until apparent ovulation. Jugular blood samples were collected every other day from Day 2 through 6 days after mating at posttreatment estrus, when uteri were flushed nonsurgically. The ovulatory follicle, which was followed retrospectively and had been observed longer (14 ± 1 vs. 8 ± 1 days), was larger in PF (17.2 ± 0.5 mm) than in GF (14.5 ± 0.6 mm; p < 0.01). Mean estradiol-17β (E2) in jugular plasma during the 6 days before estrus was higher in PF than in GF (5.7 ± 0.4 vs. 2.6 ± 0.4 pg/ml;p < 0.01). Recovery of oocytes/embryos was lower (70%) in PF than in GF (90%; p < 0.05). Fertilization rate (97% ) and mean numbers of accessory sperm in the zona pellucida (38 ± 12), for recovered oocytes/ embryos, did not differ with treatment. Fewer embryos had reached ≥ 16-cell stage of development in PF ( 14% ) and were good morulae (14%) than in GF (86% and 73%, respectively; p < 0.01). Death of embryos in cows with PF could be due to the effects of elevated E, on either oocyte maturation or oviductal function." @default.
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• W2040994414 date "1995-05-01" @default.
• W2040994414 modified "2023-10-02" @default.
• W2040994414 title "Effect of Persistent Follicles on Early Embryonic Losses in Beef Cows1" @default.
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• W2040994414 doi "https://doi.org/10.1095/biolreprod52.5.1129" @default.
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