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• W2041036003 abstract "A glucose amperometric biosensor based on glucose oxidase immobilized on an overoxidized polypyrrole (PPyox) platinum modified electrode, by glutaraldehyde co-crosslinking with bovine serum albumine, is described. The advantages of covalent immobilization techniques (e.g. high loading and long-term stability of the enzyme) are coupled with the excellent interferent rejection of electrosynthesized non-conducting polymers. The sensor showed an apparent Michaelis-Menten constant of 16 ± 0·8 mM, a maximum current density of 490 μA/cm2 and a shelf lifetime of at least 3 months. Ascorbate, urate, cysteine and acetaminophen at their maximum physiological concentrations produces a glucose bias in the low micromolar range. Flow-injection response was linear up to 20 mM glucose with typical sensitivy of 84·0 ± 1·5 nA/mM. The sensor was tested for glucose determination of untreated serum samples from both normal and diabetic subjects; results of amperometric assay compared well with those obtained by a standard enzymatic-colorimetric method." @default.
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• W2041036003 date "1998-01-01" @default.
• W2041036003 modified "2023-09-27" @default.
• W2041036003 title "Electrosynthesized non-conducting polymers as permselective membranes in amperometric enzyme electrodes: a glucose biosensor based on a co-crosslinked glucose oxidase/overoxidized polypyrrole bilayer" @default.
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• W2041036003 doi "https://doi.org/10.1016/s0956-5663(97)00064-x" @default.
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