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- W2041036258 abstract "A selected ion monitoring method for the determination of 6-oxo-PGF1 alpha, the stable end-product of prostacyclin, in biological fluids has been developed. In this method, biosynthetically prepared [2H6]-6-oxo-PGF1 alpha is used as internal standard. The method involves extraction, thin-layer chromatography purification and derivatization into the methyl ester, methoxime, trimethylsilyl ether derivatives by carrying out the methoximation first. Quantitative gas chromatographic mass spectrometric analysis is performed in the electron impact mode by monitoring the [M - (TMSOH + CH3O)]+ fragment ions. The use of this method in the measurement of 6-oxo-PGF1 alpha in serous fluids and in incubation media of serous tissues is described." @default.
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- W2041036258 date "1980-11-01" @default.
- W2041036258 modified "2023-09-24" @default.
- W2041036258 title "Quantitative determination of 6-Oxo-PGF1α in biological fluids by gas chromatography mass spectrometry" @default.
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- W2041036258 doi "https://doi.org/10.1002/bms.1200071119" @default.
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