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• W2041036633 abstract "A new forward primer, Mb-F, was designed to improve the sensitivity and reproducibility of the Mycoplasma bovis-specific PCR developed by Ghadersohi et al. [Vet. Microbiol. 56 (1997) 87] for testing clinical samples. A semi-nested (SN) PCR configuration was developed and this provided enhanced sensitivity and reproducibility. The detection limit of the SN PCR was in the range of 10-100cfu/ml and the correct amplicon was amplified from 9.15pg/microliter of total extracted DNA (mixture of M. bovis and bovine cellular DNA). A dot blot assay was also developed and compared with the SN PCR on a number of randomly selected milk and mucosal samples. The dot blot had the same level of detection as the SN PCR. The specificity of the SN configuration was confirmed by Southern blot analysis and automated sequencing of the PCR product. The results from the tests on the samples from cattle, together with those from sheep, provided evidence that M. bovis is host-specific and that most cattle are colonised. The assay was shown to be specific, sensitive and reproducible and could be used successfully to detect M. bovis directly from clinical material without pre-enrichment." @default.
• W2041036633 created "2016-06-24" @default.
• W2041036633 creator A5071265573 @default.
• W2041036633 creator A5073710483 @default.
• W2041036633 date "2003-02-01" @default.
• W2041036633 modified "2023-09-29" @default.
• W2041036633 title "Development of a semi-nested PCR for the improved detection of Mycoplasma bovis from bovine milk and mucosal samples" @default.
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• W2041036633 doi "https://doi.org/10.1016/s0378-1135(02)00305-x" @default.
• W2041036633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12458159" @default.
• W2041036633 hasPublicationYear "2003" @default.
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